摘要
目的探讨连翘提取物对脓毒症大鼠肝基因表达的影响。方法将90只Wistar大鼠随机分为3组，似手术组（30只）、模型组（30只）、连翘组（30），模型组采刚大鼠肓肠结扎穿孔（CLP）复制脓毒症模型，似手术组大鼠麻醉、开腹后只做盲肠翻动，不做结扎及穿孔，其余同模型组，连翘组CLP复制脓毒症模型。分别观察各组动物48、72h死亡率，模型组和连翘组于CLP术后72h后脱椎处死动物，无菌条件下切取大鼠肝脏大小约10mm×10mm×3mm肝组织。假手术组于同一天相同条件下取大鼠肝组织。用Rat Cenome2302．0芯片检测模型组／假手术组、连翘组／模型组大鼠肝组纵基因变化，并以二者荧光信号相对强度，比值〉2或〈-2筛选差异基因，通过美国国立生物技术信息中心（NCBI）数据库查询基因功能并加以分类。结果连翘组48、72h大鼠死亡率分别为30％和50％，模型组48、72h大鼠死广率分别为46．7％和70％，两组死亡率的差异均有统计学意义（P〈0．05）。CLP术后72h模型组／假手术组上调而连翘组／模型组下调的已知基因14条，模型组／假手术组下调而连翘组，模型组上调的已知基因11条，其中涉及免疫相关基因8条，代谢相关基因5条，物质运输相关基因2条，细胞黏附相关基因2条，细胞增殖、分化、凋亡相关基因4条，转录调控相关基因2条及其他基因。结论连翘提取物能降低脓毒症大鼠48、72h死亡率，能调控脓毒症时肝组织多数免疫、炎症、代谢等相关异常基因表达发生回归。
Objective To study the effects of forsythia extract on the liver gene expression levels of rats with sepsis model. Methods The 90 Wistar rats were randomly divided into three groups：sham operation group （n=30）, sepsis model group （n=30） and forsythia group （n=30）. The survival rates at 48 11 and 72 h were observed for all groups. The sepsis model and torsythia group rats were prepared by ＂CLP＂ method. 72 h later the rats were sacrificed by removed the vertebra. Under sterile conditions,cut the size of about 10 mm×10 min×3 mm rat liver tissue and placed in liquid nitrogen for use. The same with the sham operation group. The gene expression levels of livers in all groups were detected by the Applications Rat Genome 230 2.0 microarray,and the relative strength of both the fluoreseenee signal ratio〉2 or 〈-2 screening significantly different genes, by the US National Center for Bioteehnology Information （NCBI） database query gene function and classify. Results Forsythia group 48 h, 72 h rat mortality rates were 30% and 50%, the sepsis model group 48 h, 72 h rat mortality rates were 46.7% and 70%, two groups 48 h, 72 h mortality rates were compared, the differences were statistically significant （P〈0.05）. 72 hours after CLP, the genes with up-regulation in sepsis model group/sham operation group and with down-regulation in Forsythia group/sepsis model group were 14. The genes with down- regulation in sepsis model group/sham operation group and with up-regulation in Forsythia group/sepsis model group were 11. The genes involves immune-related genes 8, nletabolism genes 5, material transport two related genes, cell adhesion two related genes, cell proliferation, differentiation and apoptosis related genes 4, transcriptional regulation genes 2and other related gene. Conclusion Forsythia can reduce the 48, 72 h mortality of rats with sepsis and can regulate abnormal sepsis liver genes whieh associated with tissue immunity, inflammation, metabolism occur regression expression.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2016年第5期352-356,共5页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
天津市科委重点基金（10JCZDJC18900）
天津市科委一般项目（12JCYBJC17600）
天津市卫生计生委攻关项目（13KG104）
天津市卫生计生委科技基金重点项目（2015KR16）
天津市中医药管理局基金（2015044）
国家中医药管理局“十二五”重点专科建设项目
王今达学术思想研究室项目
关键词
植物
药用
连翘
脓毒症
基因表达
Plants, medicinal
For SYTHIA S USPENSA
Sepsis
Gene expression
