摘要
从大果紫檀心材提取物对木腐菌的细胞膜通透性、有氧呼吸等方面入手,探讨大果紫檀心材提取物对木腐菌抑制的生理生化机制及可能的作用靶点。结果表明:大果紫檀心材提取物能够破坏木腐菌的细胞膜,使其通透性变大,从而使其胞内蛋白透出;大果紫檀心材提取物还能抑制木腐菌的有氧呼吸,使其能量代谢出现异常,从而导致生命活动变弱甚至停止;褐腐菌分泌纤维素酶的酶活随提取物处理浓度的升高而呈逐渐降低的趋势;同时提取物的作用靶位较多。研究结果有利于深入地了解大果紫檀心材的耐腐机理,为大果紫檀木材加工剩余物的合理利用提供科学依据。
The enzyme activity of cellulose,cell membrane permeability and oxygen breath were detected to explore the physiological and biochemical mechanism of the extracts of Pterocarpus macrocarpus heartwood as well as their possible targets.The results showed that the extracts could destroy the cell membrane of the wooddecaying fungi to strengthen the permeability and make the intracellular protein revealed. Additionally,the extracts had oxygen consumption inhibition effect,leading to abnormal energy metabolism.As a result,the life activity of wood-decaying fungi became weak even stopped.The inhibition activity of cellulase which was secreted by G. trabeum increased with the rise of the extracts＇ concentration.The possible targets were more as well.The result is beneficial to the understanding of the mechanism of heartwood against wood-decaying fungi providing scientific basis for the rational use of processing residues of Pterocarpus macrocarpus.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2016年第2期327-331,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
福建省植物资源化学与材料技术开发平台建设经费资助项目(K8412001A)~~
关键词
大果紫檀
提取物
木腐菌
生理生化机制
Pterocarpus Macrocarpus
extracts
wood-decaying fungi
physiological and biochemical mechanism
