摘要
目的为证实溪黄草单味药具有体外抗乙型肝炎病毒活性提供重要的科学依据。方法将溪黄草50%乙醇提取物配置成一定药物浓度,拟采用HepG2.2.15细胞模型进行细胞毒试验和乙型肝炎表面抗原(HBsAg)、乙型肝炎e抗原(HBeAg)酶联免疫检测。结果半数细胞抑制时其浓度为4.275 mg/mL,溪黄草粗提物对HBsAg的半抑制浓度(IC50)为2.250 mg/mL,对HBeAg的IC50为2.150 mg/mL,治疗指数小于2。结论溪黄草粗提物具有抑制体外乙型肝炎病毒的作用。
Objective To provide an important scientific basis for verifying the in vitro anti-HBV activity of single herb Rabdosia Serra. Methods 50%ethanol extract of Rabdosia Serra was dispensed as certain concentrations. The HepG2.2.15 cel-lular model was adopted to conduct the cellular toxicity test and HBsAg and HBeAg enzyme-linked immunosorbent ssay（ELISA）. Results IC50 was 4.275 mg/mL,IC50 of crude extract of Rabdosia Serra on HBsAg was 2.250 mg/mL,which on HBeAg was 2.150 mg/mL,the therapeutic index（TI）＆lt;2. Conclusion The crude extract of Rabdosia Serra has the effect for in vitro inhibiting HBV.
出处
《现代医药卫生》
2016年第10期1465-1467,共3页
Journal of Modern Medicine & Health
关键词
溪黄草
肝炎病毒
乙型
细胞培养技术
植物
药用
细胞毒性试验
免疫
Isodon striatus
Hepatitis B virus
Cell culture techniques
Plants,medicinal
Cytotoxicity tests,immunologic
