摘要
目的观察基质金属蛋白酶7（MMP-7）、细胞外基质金属蛋白酶诱导因子（EMMPRIN）蛋白及m RNA在类风湿关节炎（RA）患者外周血中性粒细胞中的表达,探讨其与RA相关性及意义。方法采集2014年1月~2015年5月本溪市中心医院RA患者（RA组）及健康体检者（NC组）外周血中性粒细胞,采用Western blot及q RTPCR分别检测MMP-7及EMMPRIN在蛋白及m RNA水平表达,采用酶联免疫法检测血清炎性因子表达,并探讨其意义。结果 RA组中MMP-7及EMMPRIN蛋白表达明显高于NC组（t=7.752,6.627,P〈0.01）。RA组中MMP-7 m RNA及EMMPRIN m RNA表达明显高于NC组（t=5.654,P〈0.01;t=3.531,P〈0.05）。Spearman相关性分析显示,外周血中性粒细胞MMP-7及EMMPRIN蛋白表达外周血与CRP、RF、PGE2、IL-8水平呈正相关（P〈0.01或P〈0.05）。结论 RA患者外周血MMP-7及EMMPRIN在蛋白及m RNA水平均升高,与RA发展具有正相关性。
Objective To observe the expression of MMP-7,EMMPRIN protein and m RNA in peripheral polymorphonuclear leukocytes of RA patients and to explore the relationship and meaning with rheumatoid arthritis.Methods The peripheral blood neutrophil was collected from RA patients（group RA） and healthy subjects（group NC）from January 2014 to May 2015 in Benxi Central Hospital.Western blot and q RT-PCR were respectively used to tested the expression level of MMP-7,EMMPRIN protein and m RNA.Enzyme linked immunosorbent assay was used to tested the expression of serum inflammatory cytokines and the meaning was explored. Results The expression of MMP-7 and EMMPRIN protein in group RA was obvious higher than that of group NC respectively（t =7.752,6.627,P 0.01）.The expression of MMP-7 m RNA and EMMPRIN m RNA protein in group RA was obvious higher than that of group NC respectively（t=5.654,P0.01;t=3.531,P0.05）.Spearman related analysis showed that MMP-7 and EMMPRIN protein in peripheral polymorphonuclear leukocytes had statistical positive relationship with CRP,RF,PGE2 and IL-8 levels（P 0.01 or P 0.05）. Conclusion All of MMP-7,EMMPRIN protein and m RNA level in peripheral polymorphonuclear leukocytes increase and has positive relationship with the progress of rheumatoid arthritis.
出处
《中国当代医药》
2016年第12期46-48,共3页
China Modern Medicine
