摘要
目的:建立药材香青兰中熊果酸和齐墩果酸HPLC含量同时测定方法。方法:ZORBAX C18色谱柱(4.6mm×250mm,5um),检测波长:220nm,流速:0.6mL/min,柱温:25℃,流动相:甲醇-水-冰醋酸-三乙胺(85∶15∶0.04∶0.08)。结果:所测成分熊果酸进样量在0.1017μg^2.5425μg(r=0.9994)范围内与峰面积呈良好线性关系;齐墩果酸进样量在0.3063μg^7.6575μg(r=0.9996)范围内与峰面积呈良好线性关系。加样回收率分别是98.07%(RSD=0.66%)和99.41%(RSD=1.02%)。结论:本文所采取的方法准确可靠,适用于香青兰中熊果酸和齐墩果酸的含量的同时测定。
Objective：To establish method to simultaneous determination the content of ursolic acid and oleanic acid in dracocephalum moldavicum L.Methods：HPLC was carry out on a ZORBAX C18column（4.6×250 mm,5um）with mobile phase methanol-water-acetic acid-triethylamine（85∶15∶0.04∶0.08）,using flow rate of 0.6mL/min,the column temperathre was set at 25℃,the detection wavelength was at 220 nm.Results：The linear range of Ursolic acid was 0.1017μg^2.5425μg（r=0.9994）,the linear range of oleanic acid was 0.3063μg^7.6575μg（r=0.9996）,within this range the linear relationship was good.The average recoveries were 97.12%（RSD=1.02%）and 97.28%（RSD=0.97%）,respectively.Conclusion：This method is rapid,accurate and reliable;It could be used to simul taneous determine the content of ursolic acid and oleanolic acid in dracocephalum moldavicum L.
出处
《陕西中医》
2016年第5期622-624,共3页
Shaanxi Journal of Traditional Chinese Medicine
关键词
熊果酸/中药分析化学
齐墩果酸/中药分析化学
色谱法
高压液相
@香青兰
Ursolic acid/analytical chemistry（TCD）
Oleanolic acid/analytical chemistry（TCD）
Chromatography
high pressure liquid
@Xiangqing Lan
