摘要
利用扩增片断长度多态性 (Amplifiedfragmentlengthpolymorphism简作AFLP)分子标记技术研究了 2 3个水稻品种的遗传多样性。从 4 9对AFLP引物中筛选出 4对多态性高、分辨能力强的引物 ,4个AFLP引物组合分别扩增出 4 3、 37、 4 9、 33条多态性带 ,平均每个引物组合扩出4 0 5条多态性带 ,4个引物组合共扩增出 2 4 6条带。利用AFLP数据进行聚类分析 ,将 2 3个品种聚为 10个类群。结果表明 ,AFLP在水稻类群划分的应用是可行的。
AFLP marker technique was used to study the diversity of 23 rice species Four pairs of primers with high polymorphism and powerful distinctiveness were selected from 49 pairs of primer Four AFLP primer combinations revealed 43,37,49 and 33 AFLP polymorph bands respectively,with an average of 40 5 bands produced per primer pair Using 4 primer combinations could produce 246AFLP bands The cluster analysis based on AFLP dada showed that 23 rice species could be divided into 10 groups accorded with pedigree The current Study indicated that it is practical to group rice species with AFLPs
出处
《中国农学通报》
CSCD
2002年第4期1-3,6,共4页
Chinese Agricultural Science Bulletin
基金
云南省国际科技合作项目 (98C0 10 )。
关键词
AFLP技术
检测
水稻
遗传多样性
品种
聚类分析
Amplified fragment length polymorphism
Rice species
Genetic diversity
cluster analysis
