摘要
对枯草芽胞杆菌2012进行诱变,提高抗菌物质的产量,并提取抗菌物质。用紫外线及亚硝基胍协同诱变处理对数生长中期的枯草芽胞杆菌2012(B.s.2012),在大肠埃希菌为敏感菌的生物鉴定板上筛选产量提高的菌株。比较用乙酸乙酯法、80%乙醇法和甲醇法提取抗菌物质的优劣。正交试验优化甲醇提取法。突变株B.s.2012-21和B.s.2012-28的抑菌圈直径比原菌株分别增大84.62%和40.38%。甲醇法提取的得率最高。正交试验结果表明,提取p H为2.0,菌株发酵时间为36 h,4℃处理时间为36 h时,抗菌物质的得率最高。获得抗菌物质产量大幅提高的B.s.2012-21突变菌株。甲醇法是提取抗菌物质得率最高的方法。
B.subtilis 2012 was mutagenized to increase the production of antibiotic substances,and extracted its antimicrobial substances.The B.s.2012 in mid-logarithmic growing period was mutagenized cooperatively treated with NTG and UV,and screened the strains with increased production on a bio-identifying plate with sensitive microbes.The good and bad of the extracted antibiotics were compared using the extraction with ethyl acetate method,80% ethanol and methanol method.And optimized the methanol method with orthogonal experiment.The results showed that diameter of the inhibition zone of mutant strains B.s.2012-21 and B.s.2012-28 increased by 84.62% and 40.38% respectively as compared with the original strain.The extraction rate by methanol method was the highest.The results of orthogonal experiment showed that,when p H at 2.0,the fermentation time was 36 h and treated at 4 ℃,the recovery rate of antibiotics was the highest.This study obtained a mutant strain B.s.2012-21 with antibiotic substance production increased by big margin and methanol method to extract the antibacterial substances was the best method at the highest extraction rate.
出处
《微生物学杂志》
CAS
CSCD
2016年第2期39-44,共6页
Journal of Microbiology
基金
武汉设计工程学院(原华中农业大学楚天学院)重点科研基金项目(K201403)
关键词
枯草芽胞杆菌
抗菌物质
紫外诱变
NTG诱变
提取
Bacillus subtilis 2012
antibacterial substances
UV mutation
NTG mutation
extraction
