摘要
为探究脱油后灵芝孢子粉废料的再利用价值,对其中的粗多糖的提取工艺进行优化。以单因素实验结果为基础,以液料比、提取温度、提取时间为自变量,粗多糖得率为响应值,采用Box-Behnken实验设计方案,研究各自变量及其交互作用对粗多糖得率的影响,提取温度与液料比、提取温度与提取时间交互作用较明显,液料比与提取时间交互作用相对不明显;并确定灵芝孢子粉废料粗多糖提取的理论最佳条件为液料比25.00∶1,提取温度100.00℃,提取时间3.00 h,在此条件下粗多糖理论得率达13.320%。实际提取条件为液料比25∶1,提取温度100℃,提取时间3 h,实测值为13.204%,理论值与实测值误差小于1%。利用响应面方法优化的工艺参数可用于灵芝孢子粉废料粗多糖的提取,获得最大得率。
To explore the recycling value of defatted Ganoderma lingzhi spore scarp, the extraction technology of crude polysaccharides from defatted Ganoderma lingzhi spore scarp was optimized.On the base of the single factor investigations ,the ratio of water to material, the extraction temperature and extraction duration were studied with the Box-Behnken experimental design, and their interactions on the yield of crude polysaccharides from defatted Ganoderma lingzhi spore scarp can be concluded as follows,, interactions of time and temperature, temperature and ratio of solvent to material were remarkable, but the interaction of ratio of solvent to material and time was unremarkable.The optimum conditions were the ratio of solvent to material of 25.00 ： 1, temperature of 100.00 ℃ and time duration of 3.00 h.On these conditions,the yield of crude polysaccharides was up to 13.320%. The actual conditions were the ratio of 25 ：1, temperature of 100 ℃ and time duration of 3 h, and the actual extraction yield was 13.204.%.Error between theoretical yield and actual yield was less than 1%. It was maximal extraction yield of crude polysaccharides from defatted Ganoderma lingzhi spoer scarp with optimized process parameters.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第11期217-221,共5页
Science and Technology of Food Industry
基金
国家科技支撑计划课题(2013BAD16B08-02)
关键词
灵芝孢子粉废料
粗多糖
响应曲面法
提取工艺
defatted Ganoderma lingzhi spore scarp
crude polysaccharides
response surface methodology
extraction process
