摘要
以10 g栾树叶粉末为原料,在单因素试验基础上,采用响应面法优化栾树叶总多酚超声波辅助提取工艺,以乙醇体积分数、液料比、提取时间、提取温度、超声波功率为因子,总多酚质量分数、总多酚得率为响应值,得到优化提取条件为乙醇体积分数72%、液料比25∶1(m L∶g)、提取时间15 min、提取温度60℃、超声波功率200 W以及提取3次。在此条件下,栾树叶粗提物总多酚质量分数为61.26%、总多酚得率为3.41%。抗氧化实验结果表明:栾树叶粗提物清除1,1-二苯代苦味酰基(DPPH)自由基能力显著高于BHT和Vc,IC_(50)值为103.78 mg/L;栾树叶粗提物的铁离子还原能力与BHT相当,明显高于Vc。
Ultrasound assisted extraction（ UAE） was applied for the extraction of polyphenols from Koelreuteria paniculata Laxm.leaves using response surface methodology（ RSM） to optimize experimental conditions with l0 g sample powder as raw material.The optimal conditions were ethanol concentration 72 %,liquid to solid ratio 25 ∶ 1（ m L ∶ g）,time 15 min,temperature 60 ℃,ultrasonic power 200 W and extracting 3 times. Under the conditions,the responses of total phenolics and yield of total phenolics were 61. 26 % and 3. 41 %,respectively. The 1,1-diphenyl-2-picrylhydrazyl（ DPPH） radical scavenging ability of extracts from K. paniculata Laxm. leaves powder was significant higher than that of butylated hydroxytoluene（ BHT） and Vc. And the IC_（50） value was 103. 78 mg / L. The ferric reducing antioxidant power（ FRAP） of the extracts was as high as that of BHT and higher than that of Vc.
出处
《生物质化学工程》
CAS
北大核心
2016年第3期27-34,共8页
Biomass Chemical Engineering
基金
中央高校基本科研业务费专项基金(2015XKZD08)
关键词
栾树
总多酚
响应面
超声波
抗氧化
Koelreuteria paniculata Laxm
polyphenols
response surface methodology
ultrasonic
antioxidants
