摘要
本研究基于高通量测序技术对感染猪瘟兔化弱毒株(C株)后0h,12h,24h,30h,36h,48h的家兔脾脏组织进行测序,以家兔基因组作为参考,筛选感染后各时间点和对照组(0h)之间的差异基因。利用Uniprot和NCBI数据库查找各组中变化水平显著的前10个差异基因的生物学功能。结果发现,感染后12h,24h,30h的前10个差异基因只有少数与炎症、免疫、凋亡等相关;感染后36h,48h的前10个差异基因完全相同,其中B2M、RLA-DR-ALPHA、CD74和IGJ参与抗病毒过程。GO功能注释结果显示:感染后各时间点的差异基因都和免疫、代谢、调控途径紧密相关。KEGG通路富集分析发现,感染后24h的差异基因富集到RIG-I样受体信号通路,感染后30h的差异基因富集到黏着斑和细胞外基质-受体相互作用通路,感染后36h和48h的差异基因共同富集到的通路有20个,其中蛋白酶体,溶酶体,核糖体,趋化因子信号通路,B细胞受体信号通路,抗原加工提呈通路和FcγR介导的吞噬通路与抗病毒相关。本研究建立的家兔感染C株后脾脏组织的差异表达基因数据库,将为进一步阐述家兔适应C株的分子机制奠定基础。
In the current study,rabbit spleen was analysed at 12 h,24h,30 h,36h,and 48hpost-infection with hog cholera lapinized virus（C strain）using high-throughput sequencing.The rabbit genome was used as a reference to identify differentially expressed genes（DEGs）at different time points post-infection.The top 10 DEGs were filtered based on significance,and we searched for their biological functions through the Uniprot and NCBI databases.The former three time points have 10co-expressing genes,many of which have a relationship to immunity and inflammation.The latter two time points for the top 10 DEGs are identical,and B2 M,RLA-DR-ALPHA,CD74,and IGJ are involved in the antiviral immune response.GO functional annotation revealed that in biological processes at each time point,except for 24hpost-infection,the immune response has the most terms,followed by metabolism and regulation.According to the KEGG database,the DEGs for 24hpost-infection were enriched for the RIG-I-like receptor signaling pathway and the DEGs for 30hpost-infection were found to have a focal adhesion and ECM-receptor interaction pathway.Moreover,the DEGs for 36 hand 48hpost-infection have seven identical pathways,of which were directly or indirectly related to the antiviral response.These pathways included the proteasome,lysosome,ribosome,chemokine signaling pathways,B cell receptor signaling pathway,antigen processing,and presentation pathway,and the Fc gamma R-mediated phagocytosis pathway.These results provide novel insight into the gene expression in rabbit spleens post-infection with C strain,and provide a theoretical basis for further understanding of the molecular mechanisms by which rabbits adapt to infection with C strain.
出处
《病毒学报》
CAS
CSCD
北大核心
2016年第3期316-323,共8页
Chinese Journal of Virology
基金
内蒙古"草原英才"工程专项资金(项目号:10803)
关键词
猪瘟兔化弱毒株
家兔脾脏
转录组分析
Hog cholera lapinized virus
Rabbit spleen
Transcriptome analysis
