摘要
以"瑞星五号"番茄为试材,采用分子标记的方法,开发了番茄成熟突变体rinSCAR标记。结果表明:该标记可在正常成熟番茄材料中扩增出580bp的目的条带,而在成熟突变体rin基因组中无法扩增出该目标条带。在已知表型的"瑞星五号"番茄后代中进行验证,Prin能高效鉴定出成熟突变体rin植株。
Taking ‘Ruixing No.5＇tomato as test materials,using molecular marker method,agene-specific scar molecular mark Prin for rin was developed.The results showed that this mark could amplify 580 bp of DNA sequence in normal mature tomato materials,but not in genome of mature mutant rin.Then,the marker was used in known phenotypic offsprings of‘Ruixing No.5＇,and the result suggested Prin could effectively identify ripening mutant.
出处
《北方园艺》
CAS
北大核心
2016年第10期104-107,共4页
Northern Horticulture
基金
山东省良种工程农业生物资源创新利用研究资助项目(PTBR2013)
青岛市民生计划资助项目(13-1-3-3-nsh)
青岛农业大学高层次人才科研基金资助项目(663-1115041)
山东省自然科学基金资助项目(ZR2010CM047
ZR2014CQ034)
国家级大学生创新创业计划资助项目(201410435036)
