摘要
目的观察丹宁酸固定后续处理对改善丹宁酸交联牛颈静脉贮存液稳定性,和对血管热稳定性、抗钙化能力、生物力学性能及纤维结构的影响。方法新鲜牛颈静脉分别经戊二醛固定(戊二醛组)、丹宁酸交联(丹宁酸组)及丹宁酸交联后续处理(实验组),采用大鼠皮下植入模型测定钙含量、热皱缩温度、断裂强度和断裂伸长率、以及组织病理学检查等指标进行评价。结果研究组所有指标均优于戊二醛组(P<0.05);与丹宁酸组比较,实验组的贮存液颜色不再变化,热皱缩温度有所降低,21 d钙含量略升、60 d钙含量略低(P<0.01),两组材料力学强度结果无统计学差异(P>0.05),且两组的胶原纤维和弹力纤维均保持较好的完整性。结论丹宁酸交联后处理可提高贮存液的稳定性,使牛颈静脉血管的柔软性有所恢复,同时保持了血管抗钙化能力、组织强度和纤维结构的完整性。
Objective To investigate effects of post-treatment on stability of storage solution/thermostability, anticalcification, the biomechanics and fibred structures of tannic acid fixed bovine jugular vein （BJV）. Methods Fresh bovine jugular vein were treated with glutaraldehyde （Glut group） , tannic acid （TA group） , and post-treatment on TAfixed （Study group）. Calcium level was measured by implanting BJVs subcutaneously into the rats. The shrinkage temperature, tensile strength, elongation at break and histological changes were evaluated as well. Results Compared with Glut group,all results were better in Study group （P〈0.05）. Compared with TA group, in the Study group, color of storage solution did not change, shrinkage temperature and 60-days＇ calcium level decreased, 21-days＇ calcium level increased （P〈0.O1 ）; biomechanical properties were not statistics difference between TA group and Study group（P〉0.05） , and collagen fibers and elastic fibers were maintained well in TA group and Study group. Conclusion Post- treatment on TA-fixed Bovine Jugular Vein can improve stability of storage solution and BJVs elasticity, and do not change anti-calcification, biomechanical properties and fibred structures of TA-fixed BJVs.
出处
《中国比较医学杂志》
CAS
北大核心
2016年第5期87-92,86,共7页
Chinese Journal of Comparative Medicine
关键词
牛颈静脉
丹宁酸
钙化
生物力学
Bovine jugular vein
Tannic acid
Calcification
Biomechanics
