摘要
目的:探讨芍药苷(Paeoniflorin)对HepG2肝癌细胞凋亡的诱导作用,并考察其作用机制。方法:用不同浓度芍药苷(0.5,2mg/mL)对HepG2肝癌细胞进行培养,采用MTT法检测细胞活力,酶标法检测Caspase 3活性,western blot法检测NF-κB信号通路相关蛋白表达。结果:随着给药浓度的增加,芍药苷能逐渐降低HepG2肝癌细胞活力,在48小时抑制率最高;并能提高Caspase 3活性,抑制细胞核内NF-κB p65磷酸化,IκBα磷酸化,从而促进细胞凋亡。结论:芍药苷可能通过NF-κB信号通路诱导HepG2肝癌细胞凋亡,达到抗肿瘤效果。
Objective： To explore apoptotic induction effects of paeoniflorin on hepatoma carcinoma cells （HepG2）, and to investigate its mechanism. Methods： HepG2 cells were cultivated with different concentrations of paeonffiorin （0.5, 2 mg/mL）, cell viability was detected by MTT method, Caspase 3 activity measured by enzyme linked immunosorbent assay and the expression of NF-κB signaling pathway related protein by western blot method. Results： As the concentrations of the drug increased, paeoniflorin could decrease the viability of HepG2 cells gradually, and the inhibition rate reached the highest within 48 hours; it increased Caspase 3 activity, inhibited the phosphorylation of NF-κB p65 and IκBα, thereby to promote cellular apoptosis. Conclusion： Paeoniflorin could obtain anti-tumor effects by inducing the apoptosis of HepG2 cells via NF-κB signaling pathway.
出处
《西部中医药》
2016年第5期23-25,共3页
Western Journal of Traditional Chinese Medicine
