摘要
目的探讨低氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)诱导分化的骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)复合纳米羟基磷灰石(nano-hydroxyapatite,nano-HA)人工骨修复兔桡骨缺损的效果。方法取兔胫骨的骨髓分离培养并鉴定BMSCs;制备携带HIF-1α的慢病毒表达系统,感染BMSCs后与nano-HA共培养得到HIF-1α-e GFP/BMSCs/nano-HA人工骨材料。将30只2月龄新西兰大白兔随机分为3组,每组10只,均通过手术截骨后制成桡骨骨缺损模型,实验组填充HIF-1α-e GFP/BMSCs/nano-HA人工骨材料,对照组填充BMSCs/nano-HA,空白组不填充任何材料。于术后4、8、12周摄X线片观察骨缺损处,并于术后12周获得兔桡骨的大体标本和病理切片,比较桡骨缺损的愈合情况。结果经鉴定,分离培养得到的BMSCs形态良好,高表达CD90、CD105;所有实验动物的桡骨中段缺损模型均构建成功,通过对实验兔的大体标本、X线片及病理切片进行比较分析,实验组和对照组的骨缺损均有所修复,但实验组的新骨形成量更大,骨缺损修复能力优于对照组,空白组骨缺损区未能得到修复。结论 HIF-1α诱导分化的BMSCs与nano-HA复合制成的HIF-1α-e GFP/BMSCs/nano-HA复合人工骨具有良好的骨缺损修复能力,可能成为一种理想的骨缺损修复材料。
Objective To investigate the effect of the artificial bone which composed by hypoxia-inducible factor-1α（HIF-1α）-induced bone marrow mesenchymal stem cells（BMSCs） and nano-hydroxyapatite（nano-HA） in repairing rabbit radius defect. Methods The BMSCs were isolated from the tibia of rabbits, cul-tured and identified. The lentivirus of HIF-1α was prepared and HIF-1α-e GFP/BMSCs/nano-HA artificial bonematerial was produced by co-culturing BMSCs infected with HIF-1α lentivirus and nano-HA. Thirty New Zea-land white rabbits were divided into 3 groups at random. The radial bone defect model was established by surgi-cal osteotomy. The radius defects in experimental group were filled with HIF-1α-e GFP/BMSCs/nano-HA, thosein control group with BMSCs/nano-HA, and those in blank group with nothing. The bone defect was observed at4 th, 8th and 12 th week after operation by X-ray examination, and the healing of radial defect was comparedgrossly and pathologically at 12 th week after operation. Results The BMSCs had good form and high expres-sion of CD90 and CD105. The radial bone defect models were successfully constructed. The bone defects in ex-perimental group and control group healed to some extent, but the new bone formation in the experimental groupwas greater, and the repair ability was better than in the control group. Conclusion The HIF-1α-e GFP/BM-SCs/nano-HA composite artificial bone processes a good ability to repair the bone defect, and is expected to be-come an ideal material for repairing bone defect.
出处
《骨科》
CAS
2016年第3期201-206,212,共7页
ORTHOPAEDICS
基金
深圳市科技研发资金项目(CXZZ20140813160132596,JCYJ20130401112820839)
关键词
低氧诱导因子
间充质干细胞
羟基磷灰石
骨代用品
Hypoxiainducible factor
Mesenchymal stem cells
Hydroxyapatites
Bone substitutes
