摘要
目的：建立超微戊己丸中多成分含量测定方法。方法：采用高效液相色谱法（HPLC）测定,色谱柱：Gemini C18柱（250mm×4.6mm,5μm）,流动相：乙腈-水,采用梯度洗脱,流速：1.0mL/min,柱温：30℃,检测波长：220、274nm,进样量：20μL。结果：盐酸小檗碱、吴茱萸碱、芍药苷的线性范围分别为40.0-160.0μg/mL,10.0-50.0μg/mL,30.0-140.0μg/mL范围内具有良好的线性关系,超微戊己丸中盐酸小檗碱、吴茱萸碱、芍药苷的平均含量分别为18.025、6.842、12.365mg/g;结论：双波长HPLC法同时测定超微戊己丸中3种成分的含量,方法灵敏,结果准确,重现性好。
Objective：To establish the determination method of multiple component content in Ultramicro Wuji pills.Methods：The determination was performed by HPLC on a Gemini C18column（250mm×4.6mm,5μm）with the mobile phase of acetonitrilewater at a flow rate of 1.0mL/min.The column temperature was 30℃.The detection wavelengh was set at 220,274 nm and the injection volumn was 20μLResults：Berberine hydrochloride,evodia rutaecarpa alkali,paeoniflorin had a linear range in the contents of40.0-180.0μg/mL,10.0-50.0μg/mL,30.0-140.0μg/mL.The average content of the three components was 18.025、6.842、12.365mg/g.Conclusion：The method was simple,reliable and accurate in the Simultaneous determination of three components in Ultramicro Wuji pills by Double wavelength HPLC.
出处
《亚太传统医药》
2016年第11期42-43,共2页
Asia-Pacific Traditional Medicine
基金
湖南省教育厅科学研究项目(13C675)
湖南中医药大学中药技能大学生创新实验项目(201505)
关键词
超微戊己丸
双波长
高效液相色谱法
含量测定
Ultramicro Wuji Pills
Double Wavelength
HPLC
Determination of Content
