摘要
目的监测肿瘤患者化疗过程中网织红细胞（RET）的变化情况,评估RET作为骨髓造血功能抑制与恢复早期预测指标的价值。方法肿瘤患者化疗均以21 d为1个治疗周期,其中第0天为化疗前,第1~3天采用化学药物治疗,第7~21天为化疗后。采用SYSMEX XE-4000全自动血液分析仪（简称XE-4000）检测48例肿瘤患者化疗前、后RET百分比（RET%）的变化,并与传统手工计数进行比较。同时检测外周血红细胞（RBC）、白细胞（WBC）数量。根据第10天RET%升高是否超过25%将48例肿瘤患者分为RET明显升高组（RET%升高≥25%）和RET升高不明显组（RET%升高〈25%）,分析2个组外周血RBC、WBC的变化情况。结果 XE-4000 RET%检测结果与手工计数法基本一致。肿瘤患者化疗后发生不同程度的骨髓造血功能抑制,随访显示第7天、第10天外周血RBC、WBC计数和RET%均低于化疗前（P〈0.05）;随后,RBC计数随RET%的升高而升高。RET明显升高组第21天的RBC计数结果明显高于化疗前（P〈0.05）,而RET升高不明显组第21天RBC计数虽有升高,但差异无统计学意义（P〉0.05）。化疗后RET明显升高组WBC在骨髓恢复期的早期即已升高,但波动范围较大;而RBC则稍晚升高。RET升高不明显组WBC升高时间较晚,RBC基本无变化。结论 RET计数可作为肿瘤患者化疗后反映骨髓抑制或恢复较敏感的指标。
Objective To monitor the change of reticulocyte（RET）count during chemotherapy for cancer patients,and evaluate RET count as an early indicator of bone marrow arrest and recovery. Methods Twentyone d is a treatment cycle of chemotherapy for all cancer patients in this study,while the 0th day was defi ned as prechemotherapy,chemotherapy was conducted on the 1st day to 3rd day,and the 7th day to 21 st day were as postchemotherapy. RET percentages（RET%） of 48 cancer patients before and after chemotherapy were determined by SYSMEX XE-4000 automatic hematology analyzer（XE-4000）,and compared with traditional manual counting for RET. Red blood cell（RBC）and white blood cell（WBC）counts were determined. These 48 cancer patients were classifi ed into 2 groups according to RET% on the 10 th day,high-elevated RET group（RET% increased ≥ 25%）and low-elevated RET group（RET% increased 25%）. The changes of RBC and WBC counts between the 2 groups were analyzed. Results RET% by XE-4000 was in accordance with that by manual counting. Since bone marrow arrest occurred after chemotherapy,RBC count,WBC count and RET% on the 7th and 10 th day were lower than those before chemotherapy（P〈0.05）. RBC count increased with the elevation of RET%,especially on the 21 st day,which was higher than that before chemotherapy（P〈0.05）. Although RET count increased on the 21 st day,there was no statistical signifi cance in low-elevated RET group（P〉0.05）. In high-elevated RET group,WBC count increased and fl uctuated signifi cantly during bone marrow recovery,and then RBC count increased. In contrast to high-elevated RET group,WBC count in low-elevated RET group increased later,along with slight change in RBC count. Conclusions RET count might act as an early indicator of bone marrow arrest and recovery after chemotherapy.
出处
《检验医学》
CAS
2016年第5期359-362,共4页
Laboratory Medicine
关键词
网织红细胞
红细胞
白细胞
恶性肿瘤
化学治疗
Reticulocytes
Red blood cells
White blood cells
Neoplasms
Chemotherapy
