摘要
利用溶胶凝胶法制备磁性Fe_3O_4/SiO_2复合粒子,并用-氨丙基三乙基硅烷(3-APTES)对复合粒子进行修饰,作为固定化漆酶的载体,研究了固定化漆酶适宜的催化条件.结果发现,固定化漆酶的最佳反应温度为30℃,p H为4.5,固定化酶的酶活为180 U/g,酶活回收率为68.45%.考察了固定化漆酶的热稳定性、p H稳定性和储存稳定性,与游离酶相比,固定化漆酶更加稳定,便于连续操作.将固定化漆酶用于去除废水中的2,4-二氯酚,反应12 h,去除率最高为68.35%,该固定化酶重复重复使用12次后,对2,4-二氯酚的去除率可保持在52.85%.
Magnetic silica composite particles were prepared by using sol-gel reaction. The composite particles were fun- ctionalized with 3-Aminopropyltriethoxysilane （APTES） and used to immobilize laccase. The effects of catalytic condition were optimized. The results showed that catalytic pH was 4.5, catalytic temperature was 30℃, the highest specific activity of immobilized laccase reached to 182 U/g and the activity recovery was 68.45 %. Compared with free laccase, the thermal, pH, storage stabilities of the immobilized laccase were improved significantly. The catalytic activity of the immobilized laccase was also demonstrated by the degradation of 2,4-dichlorophenol. It was found that the removal rate of 2,4-di- chlorophenol was 68.35% in about 12 hours; and after 12 consecutive cycles, the removal rate was 52.85%.
出处
《河北工业大学学报》
CAS
2016年第1期85-89,共5页
Journal of Hebei University of Technology
基金
河北省科技计划项目(13273607
13274314)
关键词
磁性复合粒子
漆酶
固定化
2
4-二氯酚
magnetic composite particles
laccase
immobilization
2,4-dichlorophenol
