摘要
目的:研究蒲公英总多糖组分及其体外抗炎活性作用,筛选得到蒲公英总多糖的抗炎活性成分。方法:采用水提醇沉法提取得到蒲公英总多糖,通过二乙氨基乙基纤维素52(DEAE-52)柱层析进行纯化,得到蒲公英总多糖的3个洗脱部位(水,0.1 mol·L^(-1)氯化钠和0.2 mol·L^(-1)氯化钠洗脱部位分别记为TMP-0,TMP-1,TMP-2)。在体外以脂多糖诱导的RAW264.7细胞模型对蒲公英总多糖的体外抗炎活性进行研究。结果:蒲公英总多糖能够降低RAW264.7细胞中炎症因子(环氧合酶-2,肿瘤坏死因子-α,白细胞介素-6和白细胞介素-1β)mRNA的表达,且呈一定的剂量依赖性。TMP-0和TMP-1能够抑制上述炎症因子mRNA的表达,具有较好的体外抗炎活性,而TMP-2在中、低剂量下抗炎作用不明显。结论:通过体外细胞模型筛选,发现蒲公英总多糖具有较强的抗炎活性,其活性部位主要为TMP-0和TMP-1,为获得具有抗炎活性的均一多糖类成分提供参考。
Objective： To study on total polysaccharides in Taraxaci Herba and its in vitro antiinflammatory activity for screening active ingredient of anti-inflammatory. Method： Water extract-alcohol precipitation method was adopted to gain total polysaccharides in Taraxaci Herba（ TMP）,and then it was purified by DEAE cellulose column to get three parts of TMP（ TMP-0,TMP-1,TMP-2）. Anti-inflammatory activity of TMP on RAW264. 7 cells induced by LPS was investigated. Result： TMP can significantly reduce the mRNA expression of inflammatory factors [cyclooxygenase-2（ COX-2）,tumor necrosis factor-α（ TNF-α）,interleukin-6（ IL-6） and interleukin^-1β（ IL^-1β） ]in RAW264. 7 cells with a dose-dependent manner. The two fractions,TMP-0and TMP-1,can significantly reduce the mRNA of these above inflammatory factors,they showed a good in vitro anti-inflammatory activity,while the anti-inflammatory effect of TMP-2 at low and middle doses was not obvious.Conclusion： TMP has a strong anti-inflammatory activity,and its main active fractions are TMP-0 and TMP-1.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第11期25-28,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
佳木斯大学科技创新团队项目(CXTD-2013-05)
