摘要
目的分析胆汁细菌培养中病原菌的分布及耐药性,为临床合理选用抗菌药物控制胆道感染提供依据。方法调查医院从2013年1月-2015年12月各科室送检的胆汁中分离出病原菌的分布及耐药性,细菌的鉴定和药敏试验采用法国生物梅里埃公司VITEK 2 Compact全自动细菌鉴定药敏分析仪。结果共分离出270株病原菌,革兰阴性菌170株,革兰阳性菌82株,真菌18株,分别占63.0%、30.4%、6.7%;主要革兰阴性菌对多数抗菌药物产生了高度耐药,肺炎克雷伯菌对亚胺培南的耐药率达21.1%;主要革兰阳性菌中的屎肠球菌和粪肠球菌也有很高的耐药性,未发现耐万古霉素和利奈唑胺菌株。结论胆汁培养中的病原菌以革兰阴性菌为主,大肠埃希菌占第一位,且多为多重耐药菌,只有防止经验用药的不合理性,根据细菌和药敏结果选用抗菌药物,才能有效控制胆道感染。
Objective To explore the distribution and the drug resistance of pathogenic bacteria from bile bacteria culture,to offer proper basis for rational use of antibiotics. Methods The distribution and the drug resistance of pathogenic bacteria from bile bacteria culture from January 2013 to December 2015 were investigated. Identification and drug sensitive test of bacteria were conducted by French Bio- Merieux VITEK 2 Compact automatic bacteria identification and drug sensitivity analyzer. Results Totally 270 strains pathogenic bacteria were isolated,in which there were 170 Gram- negative bacteria,82 Gram- positive bacteria and 18 fungus,accounting for 63. 0%,30. 4% and 6. 7%,respectively. Major Gram- negative bacteria showed high drug resistance to most antimicrobial agents. The drug resistance rate of Klebsiella pneumonia to imipenem was 21. 1%. Feces Enterococcus and manure Enterococcus in Main Gram- positive bacteria showed a high drug resistance rate. There were no vancomycin and linezolid strains. Conclusion Gram negative bacteria were the main pathogenic bacteria in bile culture,the first of which was Escherichia coli,and the most of which were multi drug resistant bacteria. Most of all showed multidrug resistance. Only to prevent the irrational use of drugs,choosing the antimicrobial agents according to the results of bacteria and drug sensitivity can effectively control the infection of biliary tract.
出处
《中国卫生检验杂志》
CAS
2016年第10期1506-1508,共3页
Chinese Journal of Health Laboratory Technology
关键词
胆汁
细菌培养
病原菌
耐药性
Bile
Germiculture
Pathogenic bacteria
Drug resistance
