摘要
目的:建立降压类中成药和保健食品中非法添加18种降压药物的液相色谱串联四级杆飞行时间质谱(HPLC-QTOF/MS)快速定性检测方法。方法:采用Agilent Eclipse plus C_(18)(50 mm×2.1 mm,1.8μm)色谱柱进行分离,以0.5%甲酸水-乙腈为流动相梯度洗脱,流速为0.2 ml·min^(-1),离子源为ESI源,正离子检测模式,对降压类中成药及保健品中非法添加的该18种化学药物进行定性检测。结果:18种化学药物的检出限分别为0.2~2.5 ng·ml^(-1),12批供试品中有1批检测到添加了利血平。结论:该方法专属性强、灵敏度高,适用于降压类中成药及保健品中非法添加该18种化学药物的定性分析。
Objective： To establish a rapid, sensitive and accurate HPLC-QTOF/MS determination method for the illegally added antihypertensive drugs in traditional Chinese medicines and healthy care products. Methods： An Agilent Eclipse plus Clscolumn （50 mm × 2.1 mm, 1.8 μm） was adopted with the mobile phase of 0.5% formic acid and acetonitrile with gradient elution. The flow rate was 0.2 ml ·min- 1. The electrospray ionization source was applied and operated in a positive ion mode. Results： The detection limit of 18 antihypertensive drugs was within the range of 0.2-2.5 ng · ml-1. Reserpine was found in one sample. Conclusion： The method is selective and sensitive, which can be used for the detection of 18 chemical medicines illegally added in antihypertensive traditional Chinese medicines and health care products.
出处
《中国药师》
CAS
2016年第6期1084-1087,共4页
China Pharmacist
