摘要
选用市售同种品牌不同年龄段的奶粉,采用莱茵—埃农氏法和高效液相色谱—示差折光检测法对其乳糖和蔗糖进行测定,分析乳糖和蔗糖的差异,并对两种分析方法的结果进行比较,为奶粉中乳糖和蔗糖的检测方法提供依据。结果表明,莱茵—埃农氏法和高效液相色谱—示差折光检测法相比,高效液相色谱—示差折光检测法测定乳糖和蔗糖含量的准确度更高,蔗糖和乳糖的线性范围均为2-12 mg/mL,相关系数分别为0.999 3、0.999 6,精密度实验结果表明,相对标准偏差分别为2.53%、3.81%,平均加样回收率分别为99.70%、101.06%。高效液相色谱—示差折光检测法结果准确、分析时间短、前处理简单,适用于快速测定奶粉中蔗糖和乳糖含量。
Lactose and sucrose in milk powder from the same commercial brand for two different age groups were assayedby the Lane-Eynon’s method and high performance liquid chromatography with refractive index detector （HPLC-RID）. Thedifferences in lactose and sucrose contents among different samples were analyzed, and the results of the two methods werecompared. It was indicated that the accuracy of the HPLC-RID method was higher than that of the Lane-Eynon’s method.The linear ranges of the HPLC method for sucrose and lactose were both 2–12 mg/mL with correlation coefficients of 0.999 3and 0.999 6, respectively. The precision, expressed as relative standard deviations, for sucrose and lactose were 2.53% and3.81%, respectively. The average recovery rates were 99.70% and 101.06%, respectively. The HPLC method had someadvantages such as high accuracy, short analysis time, simple sample pretreatment and suitability for rapid determination ofsucrose and lactose contents in milk powder.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第12期139-143,共5页
Food Science
基金
2016年度河南省高校科技创新团队项目(16IRTSTHN019)
2015年河南省教育技术装备和实践教育研究立项课题(GZS008)
河南大学民生学院2015年度大学生创新创业训练计划项目(MSCXCY2015053)
关键词
莱茵—埃农氏法
高效液相色谱—示差折光检测法
奶粉
蔗糖
乳糖
Lane-Eynon’s method
high performance liquid chromatography with refractive index detector （HPLC-RID）
milk powder
lactose
sucrose
