摘要
目的制作小鼠的坐骨神经损伤模型,在坐骨神经损伤局部应用丙泊酚治疗。观察丙泊酚对坐骨神经损伤应用丙泊酚后损伤神经的恢复以及坐骨神经相连的L4~L6脊髓神经核转录因子蛋白的表达变化规律。方法采用BALB/c小鼠120只,将所有动物随机分为3组,分为模型组,对照组,丙泊酚组;将模型组与丙泊酚组制作成单侧坐骨神经损伤模型,分为8个时间点（12、24 h,3、5 d,1、2、4、8周）进行观察。对实验动物进行神经电生理检测坐骨神经损伤后的恢复情况,并对损伤侧的坐骨神经以及坐骨神经相连的L4~L6脊髓节段,应用real time-pcr,westen-blot检测各组动物坐骨神经相应脊髓节段核转录因子的表达变化。结果 Realtime-pcr及westen-blot结果表明：对照组的L4-L6脊髓节段内,核转录因子表达较不明显。神经损伤后模型组及丙泊酚组中核转录因子在损伤后呈活化状态。而丙泊酚组的核转录因子表达12、24 h,3、5 d,1周,2周时明显低于模型组。在8周时各组核转录因子表达比较差异不明显。结论丙泊酚对周围神经损伤后与其相连的脊髓节段中炎性因子核转录因子具有抑制作用,而核转录因子的表达与炎症和凋亡有关。丙泊酚可以减轻损伤神经局部的炎症反应,利于神经损伤后的恢复与再生。
Objective Sciatic nerve injury model in mice,topical application of propofol treatment of sciatic nerve injury.To observe the effects of propofol on neural injury of the sciatic nerve injury by propofol and sciatic nerve recovery after L4-L6spinal nerve is connected with the expression of NF-KB protein.Methods We divided all the 120 BALB/c mice into 3 groups randomly,one called model group one called control group and the other called propofol group;the model group and propofol group made into models of unilateral sciatic nerve injury,is divided into 8 time points（12 h,24 h,3 d,5 d,1 W,2 W,4W,8 W）observed.The nerve electrophysiological testing was performed after sciatic nerve injury recovery of experimental animal,and on the side of injury of sciatic nerve and sciatic nerve with L4-L6spinal cord segments,the application of real time-PCR,expression of westen-blot was detected in sciatic nerve of animal spinal cord factor NF-KB.Results The Realtime-pcr and westen-blot results show that：the control group of L4-L6spinal segment,NF-KB expression was not significant.After nerve injury factor NF-KB model group and propofol group after the damage is activated.The propofol group NF-KB expression of 12 h,24 h,3 d,5 d,1 W,2 W is obviously lower than that of model group.In the 8 W group was no significant difference in NF-KB expression.Conclusion Propofol has the inhibitory effect on spinal cord segments by factor NF-KB after peripheral nerve injury,connected,and the expression of NF-KB and inflammation and apoptosis.Propofol can reduce local inflammation reaction damage nerves,conducive to the restoration and regeneration after nerve injury.
出处
《内蒙古医学杂志》
2016年第3期259-262,共4页
Inner Mongolia Medical Journal
