摘要
在水温23℃的条件下,对黑尾近红鲌（Ancherythroculter nigrocauda Yih et Wu）精子在不同浓度氯化钠溶液、不同水体以及不同离体时间情况下的活力进行了研究。结果表明,1在0～1.2%的氯化钠溶液中,氯化钠浓度小于0.5%时,随着浓度的增加,黑尾近红鲌精子快速运动时间随之延长;在0.5%氯化钠溶液中,精子快速运动时间最长（52.00±3.46 s）,与其他组别差异显著（P〈0.05）;当浓度大于0.9%时,精子未激活。2精子在蒸馏水中的快速运动时间（36.33±1.15 s）显著（P〈0.05）高于在自来水（32.00±1.00 s）和池塘水（27.67±1.52 s）中。3采用蒸馏水激活离体精子1～15 h,随着时间的延长,存活精子快速运动时间无显著差异（P〉0.05）,而精子成活率显著降低（P〈0.05）。
The spermatozoa motility of Ancherythroculter nigrocauda in different concentrations of Na Cl solution, different water bodies and different strorage time in vitro were investigated under the temperature of 23 ℃.The results showed as follows ：1In 0～1.2% Na Cl solution, the rapid movement time of the sperm of A. nigrocauda tended to longer with the increase of Na Cl concentration when it was less than 0.5%; the life span of the sperm of A. nigrocauda（52.00±3.46 s） was the longest at 0.5% Na Cl solution, which had significant difference（P〈0.05） with compared to other groups; When the Na Cl concentration was higher than 0.9%, the initiation of the sperm of A. nigrocauda was inhibited. 2 The rapid movement time of the sperm of A. Nigrocauda in distilled water（36.33±1.15 s） was significantly（P〈0.05） higher than that of tap water（32.00±1.00 s） and the pond water（27.67±1.52 s）. 3Using distilled water to activate the sperm of A. Nigrocauda in vitro 1～15 h,the rapid movement time of the survival sperms had no significant difference（P〉0.05） with the extension of time, while the survival rate of sperm significantly reduced（P〈0.05）.
出处
《湖北农业科学》
2016年第8期2062-2065,共4页
Hubei Agricultural Sciences
基金
国家科技支撑计划项目(2012BAD25B06-07)
武汉市科技计划项目(2014020202010137)
武汉市科技型中小企业技术创新基金项目(2013-补-85)
