摘要
以黑木耳多糖得率为指标,分别采用高剪切分散乳化法和酶法提取黑木耳多糖,并对提取效果进行比较。结果表明:高剪切分散乳化法提取黑木耳多糖的工艺条件为转速21 000r/min,料液比1110(m/V),时间6 min,温度80℃;酶法提取黑木耳多糖的工艺条件为酶添加量1.2%,酶解时间60min,pH 5.5,酶解温度55℃,二者得率分别为24.43%,9.29%。与酶法相比,高剪切分散乳化法提取黑木耳多糖得率高、提取时间短、操作简单。故高剪切分散乳化法是黑木耳多糖提取的一种适宜方法。
Compared the High-speed Shear Dispersing Emulsifier（HSDE）and the Enzymic Method to extract polysaccharides from Auricularia auricular by treating the Auricularia auricular polysaccharides yield as the indicator.The results showed that the optimum conditions for HSDE were：shearing speed 21 000r/min,solid-liquid ratio 1 ︰ 110（m/V）,shearing time 6 minutes and temperature80 ℃;the optimum conditions for enzymic method were：concentration of enzyme 1.2%,enzymatic hydrolysis time 60 min,pH 5.5and temperature 55 ℃.The extraction yields were 24.43% for HSDE and 9.29%for the enzymic method.Compare to the enzymic method,HSDE is more effective,time-saving and easy-to-use,which is the more appropriate method for polysaccharides extraction fromAuricularia auricular.
出处
《食品与机械》
CSCD
北大核心
2016年第4期181-186,共6页
Food and Machinery
基金
黑龙江省应用技术研究与开发计划项目(编号:GA13B202)
关键词
黑木耳
多糖
提取
高剪切分散乳化法
酶法
Auricularia auricular
polysaccharide
extraction
highspeed shear dispersing emulsifier
enzymic method
