摘要
采用薄膜-超声分散法制备蛇床子素脂质体,以包封率为评价指标,采用单因素考察和正交试验优化蛇床子素脂质体的处方和工艺条件。所得优化工艺为:胆固醇与蛋黄卵磷脂质量比为1∶2,药脂比为3∶20,Tris缓冲液p H值为8.2,成膜温度为40℃。优化后3批脂质体的平均粒径和?电位为260 nm和-28.1 m V,包封率为88%。与蛇床子素溶液相比,蛇床子素脂质体的体外释放呈现出一定的缓释特征。
The osthole liposomes were prepared by film-ultrasonic dispersion method. The formulation was optimized by single factor test and orthogonal design with entrapment efficiency as the index. The optimal parameters were as follows： the ratio of cholesterol to yolk lecithin was 1 ： 2, drug-to-lipid ratio was 3 ： 20, pH value of Tris buffer was 8.2 and hydration temperature was 40 ℃. The average particle size, ξ potential and entrapment efficiency of three batches of optimal liposomes were 260 nm, -28.1 mV and 88 %, respectively. Compared with osthole solution, the in vitro release behavior of osthole from the liposomes demonstrated a sustained-release property.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2016年第6期728-731,共4页
Chinese Journal of Pharmaceuticals
基金
河北省科技支撑项目(13272704D)
河北省教育厅重点项目(ZD2014065)
张家口市科技局资助项目(0921098D)
关键词
蛇床子素
脂质体
处方优化
体外释放
osthole
liposome
formulation optimization
in vitro release
