摘要
建立了液相色谱串联质谱法测定人血浆中的昂丹司琼(1),并考察其在健康志愿者体内的药动学。以格拉司琼(2)为内标,使用Phenomenex-C18柱,流动相为甲醇∶10 mol/L乙酸铵溶液(45∶55)。质谱采用多反应监测(MRM)模式,正离子检测,监测离子对m/z 294.1→m/z 170.2(1)和m/z 313.4→m/z 138.1(2)。1在0.1~80.0 ng/ml范围内线性关系良好,日内和日间RSD小于8.0%。18名男性健康志愿者口服1盐酸盐片后的主要药动学参数为:c_(max)(51.3±6.5)ng/ml,t_(max)(1.6±0.2)h,t1/2(4.2±0.9)h,AUC_(0→t)(257.6±64.1)ng·h·ml^(-1),AUC_(0→∞)268.0±70.1)ng·h·ml^(-1)。
A LC-MS/MS method was established for the determination of ondansetron （1） in human plasma, and its pharmacokinetics after oral administration of 1 hydrochloride tablets to healthy volunteers was investigated. Granisetron （2） was used as the internal standard. A Phenomenex-C18 column was used, with the mobile phase of methanol ： 10 mol/L ammonium acetate solution （45 ： 55）. The mass spectrometry was conducted under the modes of multiple reaction monitoring （MRM） and positive ion, with the transitions of m/z 294.1→m/z 170.2 （1） and m/z 313.4→ m/z 138.1 （2）. It was linear for 1 in the range of 0.1--80.0 ng/ml. The inter- and intra-day RSDs were less than 8.0%. The main pharmacokinetic parameters of 1 in 18 male healthy volunteers after oral administration of 1 hydrochloride tablets were as follows： Cmax （51.3±6.5） ng/ml, tmax （1.6±0.2） h, t1/2 （4.2±0.9） h, AUC0→t （257.6±64.1） ng·h·ml^- 1, AUC0-∞ （268.0±70.1） ng·h·ml^-1.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2016年第6期747-750,共4页
Chinese Journal of Pharmaceuticals
