摘要
目的探讨不同病理类型宫颈组织中TNF-α和TNFR1的表达及其与宫颈鳞癌之间的关系。方法应用实时定量RT-PCR法检测正常宫颈(正常组,48例)、宫颈上皮内瘤样病变(CIN组,47例)和宫颈鳞癌(SCC组,39例)组织中TNF-α和TNFR1、TNFR2 m RNA表达水平,采用免疫组织化学SP法检测TNF-α蛋白表达,Western blot法检测TNFR1、TNFR2蛋白表达,将TNF-α和TNFR1 m RNA表达结果与临床病理结果进行相关性分析。结果 TNF-α和TNFR1的m RNA表达水平随宫颈癌变程度的增加而升高,组间两两比较差异均有统计学意义(P<0.05),而TNFR2在各组中的表达差异无统计学意义(P>0.05);TNF-α在宫颈组织中的表达随病理程度的增加有显著升高,正常组为6.25%、CIN组为58.82%、SCC组为71.79%,组间比较差异有统计学意义(P<0.01);TNFR1蛋白表达水平随宫颈癌变程度的增加而升高,差异均有统计学意义(P<0.05),TNFR2在各组中的表达变化不明显,均与其m RNA在各组间的表达结果一致;SCC组中的TNF-α和TNFR1 m RNA的表达量与肿瘤大小、临床分期、浸润深度以及淋巴结转移情况呈正相关性,差异均有统计学意义(P<0.05),与患者年龄以及细胞分化程度无关。结论 TNF-α和TNFR1的激活与宫颈鳞癌的发生、发展相关,参与肿瘤微环境的变化,两者将有望成为治疗宫颈鳞癌的靶标。
Objective To explore the expression of TNF-α and TNFR1 in cervix tissues with different pathological types and their relationship with cervical squamous cell carcinoma（SCC）. Methods Realtime RT-PCR was performed to determine the m RNA levels of TNF-α, TNFR1, and TNFR2 in normal cervix group（n=48）, cervical intraepithelial neoplasia（CIN） group（n=47） and SCC group（n=39）. The protein levels of TNFR1 and TNFR2 in cervical tissues were assessed by Western blot. Immunohistochemistry was used to determine TNF-α expression in cervical tissues. The correlation of TNF-α and TNFR1 m RNA expression with cervical pathological types were also investigated. Results The m RNA levels of TNF-α and TNFR1 were progressively increased with the increasing grade of carcinoma（P〈0.05）, whereas the difference of TNFR2 m RNA expression in the three groups was not statistically significant（P〈0.05）. The expression of TNF-α was also progressively increased with the increasing grade of carcinoma, and the proportion of positive cells in normal cervix group, CIN group and SCC group were 6.25%, 58.82% and 71.79%, respectively（P〈0.01）. The protein level of TNFR1 was progressively increased with the increasing grade of carcinoma（P〈0.01）, whereas the TNFR2 expression was unchanged. Moreover, the positive correlations were found between TNF-α or TNFR1 m RNA levels in SCC group and tumor size, clinical stages, invasive depth, lymphatic metastasis（P〈0.05）, instead of age and differentiation degree. Conclusion The activation of TNF-α and TNFR1 may play a critical role in the initiation and progression of cervical carcinoma by contributing to the changes of cancer microenvironment, which are potential therapeutic targets of SCC.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2016年第6期497-501,共5页
Cancer Research on Prevention and Treatment
