摘要
为阐明辐射诱发兰花变异体的产生,以黄色素花虎头兰与黄蝉兰F1代杂交组培苗经60Coγ射线辐射诱变,且经6次继代培养性状稳定的优良变异株系(叶艺兰株TRIR-2、TRIR-3)和原杂交苗F1代TRIR-1(叶片全绿)为试材,通过组织培养方式研究这3种兰株在形态和生理特性方面的差异。结果显示:与原杂交苗F1 TRIR-1相比,(1)随着叶艺艺象程度增加,植株矮化越明显,艺象最明显植株的生物量、株高、叶长、叶宽和根长都下降,但根数不减,叶片数增多;(2)叶艺植株增强了光合产物可溶性糖的合成,而叶片全绿植物有利于促进蛋白质的合成;(3)植株叶艺艺象越明显,叶绿素含量越低,对光能的利用和调节能力较低,同时植株的膜脂过氧化伤害增强,但叶艺植株能通过调节抗氧化物酶活性来应对。研究表明,辐射诱发了兰花叶艺型突变体新品系,可从中选育出叶艺兰新品种。
For expounding to expound the production mutants of Cymbidium induced by irradiation, the tissue cul- ture seedlings of Cymbidium tracyanumxC, iridioides D. Don were irradiated by 60Coγ-rays, and the new mutant TRIR-2 and TRIR-3 were selected through 6-generation observation. The results showed that： （1） With the de- gree of verge line pattern leaves increased, the heights, biomass, leaf length, leaf width and root length of the seedlings decreased except the number of root, but the number of leaf increased. （2） The seedlings of verge line pattern leaves enhanced the synthesis of soluble sugar, and the all green seedlings were more inclined to the syn- thesis of soluble protein during photosynthesis. （3） When the verge line pattern leaves was more obvious, the content of chlorophyll, and the utilization and adjustment ability of light energy became lower, while the damage of membrane lipid peroxidation was more serious, but the seedlings could response by adjusting the antioxidation en- zyme activity. Research suggests that Cymbidium with verge line pattern leaves induced by irradiation, and could breed new variety.
出处
《西部林业科学》
CAS
2016年第3期38-43,共6页
Journal of West China Forestry Science
基金
国家自然科学基金项目(30160074)
云南省自然科学基金重点项目(2002C0003P)
云南省重点新产品开发项目(2012BB008)
云南农业大学园林园艺学院院级基金(2014001)
云南省昆明市科学技术局重点项目(2015-1-N-00984)
关键词
辐射
叶艺兰
形态特征
生理特性
60Coγ射线
诱变
irradiation
Cymbidium with variegation leaves
morphological characteristics
physiological charactenstics
60 Coγ-rays
induce
