摘要
目的研究饮用水中有机提取物对雌性小鼠生殖系统的毒性。方法在预处理过的大孔树脂XAD-16进行富集,分别将丰水期水源水、丰水期自来水、枯水期水源水以及枯水期自来水水中提取的有机物溶于植物油中,选择雌性小鼠40只,分为4组,每组10只,分别设为阴性对照组(给予植物油—A组)及高(5L水中有机提取物·kg^(-1)—B组)、中(2.5L·kg^(-1)—C组)、低(1.25L·kg^(-1)—D组)三个剂量组。每天经口给予各组小鼠饮用水有机提取物,连续染毒15 d,测定体重、子宫系数和雌二醇含量。结果丰水期和枯水期水源水有机提取物染毒的高剂量组和中剂量组子宫系数明显低于对照组和低剂量组;丰水期自来水有机提取物各染毒组小鼠子宫系数均明显低于对照组。丰水期和枯水期水中有机提取物染毒的低剂量组雌二醇含量明显高于对照组,高剂量组明显低于各染毒组。结论本实验中,在相同提取条件下,枯水期的饮用水中有机提取物的提取量高于丰水期,且对生殖内分泌系统的干扰作用与丰水期比较更明显。
Objective Study the toxic effects of organic extractions of drinking water on the reproductive system in female mice.Methods The source water in plentiful phase,the tap water in plentiful phase,the source water in exhausted phase and the tap water in exhausted phase were enriched by the pretreated XAD-16 macroporous resin,and their organic extractions dissolved in the plant oil respectively. 40 female mice were randomly divided into four groups（ n = 10 each） ： control（ plant oil- A group）,high dose（ 5L· kg^-1 organic extraction in water-B group）, middle dose（ 2. 5L·kg^-1-C group）, low dose（ 1. 25L· kg^-1D group）.The organic extractions were administered once a day through mouth for 15 days. The weight, the uterine organ coefficient and the content of estradiol were measured. Results The mice which were injected with the organic extractions extracted from the source water in plentiful phase and exhausted phase, their uterine organ coefficient in middle and high dose groups were lower than those in control group and low dose group obviously. The mice which were injected with the organic extractions extracted from the tap water in plentiful phase, their uterine organ coefficient in every treatment group were lower than that in control group obviously.The mice which were injected with the organic extractions extracted from the water in plentiful phase and exhausted phase, the content of estradiol in low dose group was higher than that in control group obviously,and the high dose group was lower than those in all other dose groups. Conclusion At the same condition of extraction, the content of organic extractions from drinking water in exhausted phase was higher than that in plentiful phase. In the reproductive endocrine system, the interference effect of the organic extractions in exhausted phase was more obviously than that in plentiful phase.
出处
《中国地方病防治》
CAS
北大核心
2016年第3期255-257,共3页
Chinese Journal of Control of Endemic Diseases
关键词
饮用水
有机提取物
生殖内分泌
毒性
Drinking water
Organic extracts
Reproductive endocrine
Toxicity
