摘要
实验通过一套1200 m的 PVC 管式反应器来模拟城市污水管网，利用气相色谱法、液相色谱法和454高通量测序等手段，研究了城市污水管网中产甲烷过程中的物质变化和产甲烷菌分布特性规律。结果表明，管网中甲烷含量沿程升高，说明管网中存在产甲烷菌；产甲烷菌主要包含甲烷八叠球菌属（Methanosarcina）、广古菌门中的菌属（Euryarchaeota unclassified）和甲烷杆菌科中的菌属（Methanobacteriaceae unclassified）这3种优势菌属，且在管网800～1000 m处有广古菌门中的菌属（Euryarchaeota unclassified）取代甲烷八叠球菌属（Methanosarcina）成为第一优势菌属的演替现象；管网中产甲烷可利用基质有甲酸、甲醇、甲胺、乙酸，其中乙酸为主要基质，这些基质在管网中先增加后降低的变化趋势导致了管网中产甲烷菌演替现象的发生。
A study was conducted through a 1 200 m-PVC-pipe-reactor, which was used to simulate the urban sewer system. Gas chromatography, liquid chromatography and 454 high-throughput sequencing were utilized to study the variation of substrates during the methanogenic process and the distribution characteristics of methanogens in the sewer system. The results showed that the concentration of methane increased along the sewer system, which illustrated that methanogens existed in the sewer network. The methanogens mainly contained Methanosarcina, Euryarchaeota unclassified and Methanobacteriaceae unclassified. The distinct succession which Euryarchaeota unclassified replaced Methanosarcina to be the first dominant microbial genus between 800-1 000 m of the sewer system. Formic acid, methanol, methylamine, acetic acid and hydrogen were available substrates for methanogens. Among these substrates, acetic acid was the primary substrate for methanogen. The variation trends of these substrates were first increasing and then decreasing along the length of the sewer system, which led to the succession phenomenon of methanogens in the sewer system.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2016年第6期2252-2258,共7页
Environmental Science
基金
国家水体污染控制与治理科技重大专项(2012ZX07313001)
新世纪优秀人才项目(NCET-12-1043)
陕西省创新团队项目(IRT 2013KCT-13)
关键词
城市污水管网
454
高通量测序
甲烷
产甲烷菌
演替现象
urban sewer system
454 high-throughput sequencing
methane
methanogens
succession phenomenon
