联合检测ANA、抗SSA和抗SSB对原发性干燥综合征诊断的临床意义被引量：10

Combination of ANA,anti-SSA and anti-SSB detection in the clinical diagnosis of primary Sj?gren's syndrome patients

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摘要目的调查浙江地区原发性干燥综合征患者的抗核抗体及抗核抗体谱阳性分布特点及临床意义。方法采用间接免疫荧光法和欧蒙线性免疫印迹法,回顾性分析2012年6月~2015年6月我院就诊的162例临床确诊原发性干燥综合征患者血清中抗核抗体及其抗核抗体检测谱的结果。结果 162例研究对象中抗核抗体阳性149例(占92.0%)。男7例,女155例。抗SSA阳性118例(占72.8%);抗SSB阳性72例(占44.4%);抗Ro52阳性119例(占73.4%);三者阳性检出率显著高于对照组,差异有统计学意义(P<0.05)。其他自身抗体检出率均较低。ANA分别与抗SSA、抗SSB、抗Ro52联合检测的阳性率为87.6%、65.9%、83.2%。联合检测抗SSA、抗SSB具有相对较好的特异性为57%。结论 ANA阳性患者,联合抗SSA、抗SSB判断有助于提高原发性干燥综合征诊断的特异性。 Objective To study the distribution characteristics and clinical value of antinuclear antibodies（ANA） and ANAs spectrum of the primary sj^gren＇s syndrome （pSS） patients in Zhejiang province. Methods Indirect fluorescence （IIF） and Euroline ANA Profile methods were used to detect the 162 serum specimens of pSS patients in Zhejiang Provincial People＇s Hospital from June 2012 to June 2015, then analyzed the data. Results Among the 162 pSS patients,which including 7males and 155 females. The ANA of the 149 pSS patients were positive （92.0%）. The positive rate of SSA, SSB and Ro52 antibodies were 118（72.8%）, 72（44.4%） and 119 （73.4%） respectively. The three kinds of antibodies were significantly higher than control group （P〈0.05）. The positive rate of ANA combine detection of SSA, SSB and Ro52 were 87.6%.65.9% and 83.2% respectively. The Specificity of combined detection of SSA and SSB was 57%. Conclusion In the ANA positive specimens, combined detection of SSA and SSB will improve the specificity of the pSS patients.

作者祝忠良庞珍珍刘津麟卢文静周永列

机构地区浙江中医药大学生命科学院浙江省人民医院检验科

出处《中国现代医生》 2016年第13期125-128,共4页 China Modern Doctor

基金浙江省自然科学基金资助项目(LQ14H100001)

关键词抗核抗体干燥综合征抗SSA 抗SSB Antinuclear antibody Primary sjogren＇s syndrome Anti-SSA antibody Anti-SSB antibody

分类号 R593.2 [医药卫生—内科学]

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1Wen W,Liu Y,Zhao C,et al. Clinical and serologic fea- tures of primary Sjfigren's syndrome concomitant with au- toimmune hemolytic anemia:A large-scale cross-sectional study[J]. Clin Rheumatol, 2015,34 (11.) : 1877-1884.
2Juarez M, Toms TE, De Pablo P, et al. Cardiovascular risk factors in women with primary Sjgren's syndrome:United Kingdom primary Sjgren's syndrome registry results [J]. Arthritis Care Res, 2014,66(5 ) : 757-764.
3Fatemi G, Kermani TA. Clinical significance of a positive antinuclear antibody test[J]. JAMA, 2015,314(8) : 827-828.
4Wei P,Li C,Qiang L,et al. Role of salivary anti-SSA/B antibodies for diagnosing primary SjSgren's syndrome [J]. Med Oral Patol Oral Cir Bucal,2015,20(2):e156-160.
5Biesen R, Burmester GR, Hiepe F,et al. Rheumatoid fac- tor or antinuclear antibodies as incidental finding[J]. In- ternist,2014,55 (10) : 1157-1164.
6Nasonov EL,Aleksandrova EN,Novikov AA,et al. Sys- temic autoimmune rheumatic diseases in 2013:Problems of laboratory diagnosis[J]. Ter Arkh, 2014,86 (5) : 4-9.
7干燥综合征诊断及治疗指南[J].中华风湿病学杂志,2010,14(11):766-768. 被引量：460
8Jonsson, Brokstad Theander E, SjstrSm B, et al. Autoanti- bodies present before symptom onset in primary sjogren's syndrome[J]. JAMA, 2013,6(17) : 1854-1855.
9Atisha-Fregoso Y, Rivera-Vicencio Y,Baos-Pelaez M, et al. Main causes and risk factors for hospitalisation in patients with primary Sjogren's syndrome[J]. Clin Exp Rheumatol, 2015,33 (5) :721-725.
10Sharmin S, Ahmed S,Abu Saleh A, et al. Association of immunofluorescence pattern of antinuclear antibody with specific auto antibodies in the bangladeshi population[J]. Bangladesh Med Res Counc Bu11,2014,40(2) : 74-78.