摘要
目的:探讨肿瘤坏死因子α(TNF-α)和诱导型一氧化氮合酶(iNOS)与胃炎发病及细胞凋亡的相关性。方法:选取2012年2月至2014年3月间收治的112例胃炎患者作为研究组,56例健康受检者作为对照组。所有受检者入院次日进行血压、心率、肝功能、血常规等常规项检查。研究组患者接受TNF-α、iNOS以及细胞凋亡检查,再将患者按照病情严重程度分为轻度、中度、重度3组,比较组间差异;同时采用TUNEL法检测患者胃黏膜组织的细胞凋亡情况,并采用Pearson相关性分析研究TNF-α及iNOS表达与胃炎发病和细胞凋亡的相关性。结果:(1)患者病情越严重TNF-α与iNOS水平越高,均明显高于健康对照组。(2)凋亡细胞形态、大小、染色较正常细胞均有明显不同;同时,TNF-α≥1.0 ng·mg-1的阳性细胞指数(AI)显著高于TNF-α<1.0 ng·mg^(-1),iNOS≥100个·mm^(-2)时AI显著高于iNOS<100个·mm-2,TNF-α和iNOS不同亚组之间AI差异均有统计学意义,P<0.05。(3)患者TNF-α和iNOS均与胃炎发病和细胞凋亡呈正相关,差异具有统计学意义。结论:TNF-α和iNOS的过量分泌可能引发胃炎患者胃黏膜的炎症反应,二者还可能诱导胃黏膜细胞凋亡。
Objective： To study the relationship of the expression of TNF-α,inducible nitric oxide synthase（ iNOS） and with apoptosis and gastritis. Methods： 112 patients with gastritis as a study group and 56 healthy volunteers of the same age as a control group were selected. All patients＇ liver function,blood routine,blood pressure and heart rate were examined the next day in the hospital. Next,the study group was furthermore divided into 3 groups according to illness severity,the levels of TNF-α and iNOS were compared. Apoptosis was detected by TUNEL assay. Finally,Pearson correlation analysis methods were used to analyze the correlation on iNOS,TNF-α with gastritis and apoptosis. Results：（ 1） The more severe the disease,the higher the levels of iNOS and TNF-αwould be. Moreover,both iNOS and TNF-α levels were significantly higher than those in the control group.（ 2） Apoptotic cells were significantly different from normal cells in morphology,size and TUNEL staining. At the same time,when TNF-α ≥1. 0 ng·mg（-1）,AI was significantly higher than that when TNF-α 〈 1. 0 ng·mg（-1）.When iNOS≥100 mm（-2）,AI was significantly higher than that when iNOS 〈100 mm（-2）.（ 3） Both iNOS and TNF-α levels positively correlated with the incidence of gastritis and apoptosis with significant differences. Conclusion：Inflammatory reaction of gastric mucosa for patients with gastritis may be related to the secretion of TNF-α and iNOS and they may induce apoptosis of gastric mucosal cells.
出处
《东南大学学报（医学版）》
CAS
北大核心
2016年第3期370-373,共4页
Journal of Southeast University(Medical Science Edition)
