摘要
目的:黄芪为豆科植物,它的生物活性成分种类很多,且都有不同的药理作用。黄芪内生真菌的报道很少,所以对它的研究才刚刚开始。植物次生代谢网络复杂,利用诱导子提高植物特定代谢途径的次生代谢产物的产量,对生物合成途径进行调控。诱导子分为生物诱导子和非生物诱导子,而关于诱导子的作用机理,只有一个初步的假说,没有作用机理的指导,筛选也较盲目。为了消除试验的背景噪音,无土栽培方式在植物内生真菌及根际微生物研究中,消除了土壤菌群的影响。随着新学科的产生和新技术的发展,组学技术、新一代测序技术应运而生,并在各个领域得到广泛应用。
Belonging to leguminous plants, Astragalus contained a variety of biological active ingredients with different pharmacological effects. However, endophytic fungi of astragalus were seldom reported, it was a preliminary research in this study. The plant secondary metabolism network was complex, referring to using specific metabolic pathways of plant secondary metabolites production to improve yield through elicitors, and regulation of biosynthetic pathways. Elicitor included biological elicitor and abiotic inducer. So far, there has only been a preliminary hypothesis over the mechanism of the inducer, lacking of related guidances. The process of screening was slapdash. For eliminating the ambient noise in the test, soilless cultivation in the research of endophytic fungi and rhizosphere microorganisms was utilized, all at once, to eliminate the impact of soil bacteria. With the emergence of new courses and technologies, omics technologies and updating sequencing technology were produced and widely applied in various fields.
出处
《世界科学技术-中医药现代化》
2016年第5期875-882,共8页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委面上项目(81274010):黄芪三萜皂苷类生物合成分子机制调控的研究
负责人:马伟
黑龙江省科技厅杰出青年基金项目(JC201101):黄芪SQS基因的克隆
亚细胞定位和功能研究
负责人:马伟
黑龙江中医药大学"优秀创新人才支持计划"(2012001):黄芪SQS基因的克隆
亚细胞定位和功能研究
负责人:马伟
哈尔滨市科技厅优秀学科带头人基金项目(2014RFXXJ122):诱导调控黄芪次生代谢有效成分途径及转录组学研究
负责人:马伟
关键词
黄芪
次生代谢
诱导子
无土栽培
组学技术
新一代组学技术
Astragalus
secondary metabolism
elicitor
soilless cultivation
omics technologies
updating omics technology
