摘要
为探究碳源供给策略对工厂化循环水养殖系统废水生物絮团处理的影响,以水产养殖模拟废水为研究对象,以蔗糖、乙酸钠和甘油为碳源,分别设置不同C/N比梯度(5、10和15)进行了相关研究。结果表明,各实验组均存在硝化作用和同化作用,在较低C/N比(C/N比为5)时,不同碳源硝化作用强度存在显著差异(P<0.05),但在各系统稳定阶段,氨氮去除率均达到90%以上。其中,乙酸钠为碳源时,系统达到稳定所需时间较短(4 d),并不受C/N比的影响,而另外2组随着C/N比增加,系统达到稳定所需时间逐渐减少(均由24 d减少至12 d),且氨氮去除效率逐渐提高。其次,不同碳源和C/N比对生物絮团系统碱度的影响不同,在系统稳定阶段,乙酸钠随着C/N比增加,碱度逐渐升高,且均保持较高的浓度,不适宜水产重复利用,而蔗糖和甘油相对稳定,且均低于200 mg/L。另外,结合生物絮团沉积指数可知,生物絮团的沉降性能对絮团氨氮去除效率没有显著影响(P>0.05)。在低C/N比时,不同碳源其EPS多糖含量差异不显著(P>0.05),而随着C/N比增加,不同碳源EPS多糖含量的多少及变化趋势不同。
For the trend and necessary to limit water exchange and control disease,biofloc technology（ BFT） has been widely applied in aquaculture. The carbon source supplement is the base and core of BFT,which will significantly affect the water quality,food conversion ratio（ FCR） and the immune capacity in rearing animals. To explore the influence of carbon source supply tactics on the treatment of waste water discharged by recirculating aquaculture system（ RAS）,research was conducted,based on simulated aquaculture wastewater as the research object. In addition,the sucrose,sodium acetate and glycerol were used as carbon source,and three kinds of C/N ratio（ 5,10 and 15） were evaluated for each type of carbon source respectively. Results showed that nitrification and assimilation occurred in the experimental groups. At the low C/N ratio（ C/N ratio is 5）,the nitrification intensity of different carbon source had extremely difference（ P〈0. 05）,but in the stable stage of each system,ammonia nitrogen removal rates all achieved over 90%. Moreover,sodium acetate system was quicker to reach the stablestage（ 4 d）,and less affected by C/N ratio. While with the C/N ratio increasing,the groups of sucrose and glycerol required less time to achieve stabilization（ 24 d reduce to 12 d）,and the ammonia nitrogen removal efficiencies were improved gradually（ sucrose,increasing from 87% to 99%; glycerol,from83% to 98%）. Secondly,different carbon source and C/N ratio had various effects on alkalinity of biofloc system. At system stable stage,with the increase of C/N ratio,the alkalinity of sodium acetate groups was raised slowly,and maintained high concentrations,which were not suitable for aquaculture reuse,but the groups of sucrose and glycerol were relatively stable,which were all less than 200 mg/L（ Ca CO3）. In addition,combined with biofloc sludge volume index（ SVI）,settlement performance of biofloc had no significant influence on the removal efficiency of ammonia nitrogen（ P〈0. 05）. At a low C/N ratio（ C/N ratio is 5）,the difference of EPS polysaccharide contents of three carbon source was not significant（ P〈0. 05）,and with the increase of C/N ratio,EPS polysaccharide of three carbon source had different contents and change tendencies.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2016年第6期317-323,共7页
Transactions of the Chinese Society for Agricultural Machinery
基金
国家自然科学基金青年基金项目(31402348)
'十二五'国家科技支撑计划项目(2014BAD08B09)
农业部渔业机械仪器研究所重点实验室开发基金项目(2015)
中国博士后科学基金项目(2014M551747)
