摘要
以几种园艺作物的卷须为材料研究染色体制片方法 ,发现卷须制片预处理和盐酸解离等环节的要求比根尖制片严格。利用所确立的卷须制片程序 ,验证了Cucumis属种间杂交新种Cucumis×hytivusChen&Kirkbride的体细胞染色体数为 2n =38,表明在根尖不易获取或材料珍贵稀少的情况下 ,卷须制片法是研究有卷须类植物染色体数目的有效方法。
Plant tendrils of several horticulture species were used as tissue source to research the procedure of making-slides for chromosome preparation.The condition required by tendrils was more stringent than that required by root tips in pretreatment and hydrolyzation.Through the developed procedure,chromosome number of the new species Cucumis×hytivus Chen & Kirbride was first cytogenetically confirmed to be 2n=38.When root tips cannot be obtained easily,this technique is an optimal alternate method for chromosome visualization in tendril- producing species,and especially valuable for some rare genetic germplasm.
出处
《园艺学报》
CAS
CSCD
北大核心
2002年第4期378-380,共3页
Acta Horticulturae Sinica
基金
国家自然科学基金资助项目 ( 30 170 6 44)
国家高技术研究发展计划专项经费资助项目 ( 2 0 0 1AA2 4112 3)
教育部跨世纪优秀人才培养计划项目
教育部科技研究重点项目 ( 0 10 97)
