摘要
目的通过复合酶法辅助提取短梗五加叶中金丝桃苷,以优化其提取工艺。方法采用高效液相色谱法测定金丝桃苷;采用正交实验法考察温度、α-淀粉酶、中性蛋白酶、纤维素酶对提取率的影响,确定复合酶法提取短梗五加叶中金丝桃苷的最佳条件。结果各因素主次依次为温度、α-淀粉酶、中性蛋白酶、纤维素酶。最佳条件为:纤维素酶加酶量为2%,中性蛋白酶加酶量为0.5%,α-淀粉酶加酶量为3%,温度30℃,时间10 min。短梗五加叶中金丝桃苷的提取率为0.52%。结论经复合酶处理,金丝桃苷产率较传统工艺提高,为更好开发短梗五加提供参考。
Objective To extract hyperoside from the leaves of Acanthopanax sessiliflorus by complex enzyme method,and optimize the extraction process by orthogonal experiment. Methods Hyperoside was determined by HPLC. Effects of temperature,α-amylase,neutral protease and cellulase on extraction rate were detected by the orthogonal tests,and the optimum extraction condition of hyperoside from the leaves of Acanthopanax sessiliflorus was determined by complex enzyme method.Results The main influence factor was temperature,follows by α-amylase,neutral protease and cellulase according to orthogonal analysis.The best condition was as follows： dose of cellulase,neutral protease and α-amylase was 2%,0. 5% and 3%,respectively,extract at temperature of 30 ℃ for 10 min. Under this condition,the extraction rate of hyperoside in the leaves of Acanthopanax senticosus was 0.52%. Conclusion As compared with the traditional technics,compound enzyme increases the productivity of hyperoside.
出处
《医药导报》
CAS
2016年第6期636-639,共4页
Herald of Medicine
基金
国家级大学生创新创业训练计划项目(201410193020)
关键词
短梗五加叶
金丝桃苷
复合酶
正交实验法
Leaves of Acanthopanax sessiflorus
Hyperoside
Compound enzyme
Orthogonal test
