摘要
目前以苦寒泻下、饥饱劳倦等方法诱导的脾虚动物模型存在规范性、特异性等方面问题。基因突变小鼠如APC^(min/+)、基因敲除小鼠Txnip^(-/-)、IP3R2^(-/-)、db/db小鼠以及条件性敲除Pten小鼠具有易感肿瘤、肌肉失养、肥胖、能量代谢障碍等特征,与脾虚运化失司、清阳不升、聚湿生痰和抗病能力降低相关。基于转基因小鼠结合饥饱劳倦等方法构建的病证结合脾虚模型更为客观、可信和可重复性,该类模型具有较好的转化潜力并将有助于深化脾虚证侯的本质研究。
Normative,specificity and other problems exist in spleen deficiency models caused by bitter- cold diarrhea or hunger- satiety methods. Transgenic mice,such as APCmin/＋mice,Txnip-/-mice,Pten＋/-mice,IP3R2-/-mice and db/db mice,are characterized with tumor microenvironment,muscle dystrophy,obesity,metabolic disorders and have relations with spleen deficiency and dysfunction,clear Qi not ascending,phlegm due to damp accumulation and susceptible to disease. Therefore,construction of spleen- deficiency models with transgenic mice would be objective and credible. The high reproducibility of these models has translation potential and would accelerate the research essence of combination of disease and syndrome.
出处
《辽宁中医杂志》
CAS
北大核心
2016年第6期1329-1331,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81273621
81573848)
广东省自然科学基金(2014A030313323)
关键词
脾虚证
动物模型
转基因小鼠
病证结合
spleen-deficiency
animal model
transgenic mice
combination of disease and syndrome
