摘要
前期研究发现石见穿多糖(polysaccharides from Salvia chinensis Benth.,PSSC)能有效抑制H22肝癌细胞在昆明小鼠体内的生长。在此基础上,本研究进一步探讨了PSSC对H22荷瘤小鼠免疫器官和免疫细胞的影响。通过在昆明小鼠右腋皮下注射H22肝癌细胞构建荷瘤小鼠模型。采用流式细胞术、免疫组织化学染色、酶联免疫法等实验方法评价PSSC对CD4+T细胞、CD8+T细胞、自然杀伤细胞(NK细胞)抗肿瘤免疫能力的影响。结果表明PSSC可以有效增加小鼠的脾脏/胸腺指数并促进Con A/LPS刺激的脾细胞增殖。同时,PSSC剂量依赖性地增加了荷瘤小鼠外周血单核细胞、脾脏及淋巴结中CD8+T细胞比例并提高CD8+T细胞与NK细胞在肿瘤组织中的组分含量。注射PSSC后,CD4+T细胞分泌免疫促进因子IFN-γ和IL-2的量增加,而分泌免疫抑制因子IL-4和IL-10的量减少。这一系列实验研究结果表明PSSC具有显著的抗肿瘤免疫增强活性。
Our previous study showed that polysaceharides from Salvia chinensis Benth. （PSSC） inhibited the prolifera- tion of H22 hepatoeellular carcinoma cells in Kunming mice. The current study was designed to further understand the effects of PSSC on immune organs and anti-tumor activities of immune cells on H22 tumor-bearlng mice. In this study, H22 cells were subcutaneously transplanted into fight flanks of Kunming mice. Flow eytometry analysis, immunohisto- chemical staining and enzyme-linked immunosorbent assays were performed to evaluate the immune potency of CD4 ＋ T eens ,CD8 ＋ T cens and natural killer cells （ NK cells）. The results reveled that PSSC increased spleen/thymus indexes and Con A/LPS-indueed spleenocyte proliferation in tumor-bearing mice. Ratios of CD8 ＋ T cells in peripheral blood mononuelear cells, spleens and lymph modes and numbers of CD8 ＋ T and NK cells infiltrated into transplanted tumor tissues were elevated in response to PSSC. In CD4 ＋ T cells ,PSSC provoked excretions of immunostimulatory cytokines such as IFN-T and IL-2 and suppressed those of immunosuppressive eytokines such as IL-4 and IL-10. These findings collectively suggested that PSSC were capable of enhancing anti-tumor immune response in H22 tumor-bearing mice.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第6期846-851,915,共7页
Natural Product Research and Development
基金
中南民族大学国家级大学生创新训练计划(GCX15008)
湖北省高等学校省级教学研究项目(2015195)
关键词
石见穿
多糖
H22荷瘤小鼠
抗肿瘤免疫
Salvia chinensis Benth
polysaccharides
H22 tumor-bearing mice
anti-tumor immunomodulatory effect
