摘要
目的建立益智仁Alpinae Oxyphyllae Fructus HPLC指纹图谱,并研究其抗氧化活性。方法 HPLC法建立指纹图谱,通过相似度分析、聚类分析、主成分分析进行评价,DPPH法测定其抗氧化活性。结果 HPLC指纹图谱中有24个共有峰,相似度在0.974以上,其中12、19和21号色谱峰确定为白杨素、杨芽黄素和圆柚酮。10批样品均具有抗氧化活性,但都弱于维生素C。结论该方法指纹色谱峰更多,分离度更高,而且分离时间更合适。
AIM To establish the HPLC fingerprints of Alpinia Oxyphylllae Fructus and to study the antioxidant activity.METHODS The fingerprints were established by HPLC and evaluated by similarity analysis,cluster analysis and principal component analysis.The antioxidant activity was determined by DPPH method.RESULTS There were twenty-four common peaks in the HPLC fingerprints,whose similarties were more than0.974.Among them,peaks 12,19 and 21 were identified as chrysin,tectochrysin and nootkatone,respectively.Ten batches of samples all showed antioxidant activities,which were weaker than that of vitamin C.CONCLUSION This method has the advantages of more chromatographic peaks,higher resolution and more proper separation time.
出处
《中成药》
CAS
CSCD
北大核心
2016年第6期1319-1324,共6页
Chinese Traditional Patent Medicine
基金
国家"重大新药创制"项目(2013ZX09103002014
2012ZX09304005003)
2014年四川省科技创新苗子工程项目(2014-105)
川北医学院2015年大学生开放性实验项目(2015)
关键词
益智仁
HPLC指纹图谱
抗氧化活性
相似度分析
聚类分析
主成分分析
DPPH法
Alpinia oxyphylllae Fructus
HPLC fingerprints
antioxidant activity
similarity analysis
cluster analysis
principal component analysis
DPPH method
