摘要
目的:从安息香药材中分离香脂酸类成分,并进行鉴定和初步的药效研究。方法:安息香药材采用95%乙醇提取,柱色谱方法结合制备型HPLC纯化,氢谱、碳谱及文献鉴定分离的化合物,对分离的化合物进行药效学研究。结果:从安息香药材中分离得到的成分鉴定为苯甲酸松柏酯;药效实验表明其与安息香药材有着相似功效,均能显著增加正常小鼠脑内依文思蓝含量及对D-氨基半乳糖敏化小鼠内毒素致死性攻击有一定的保护作用。结论:苯甲酸松柏酯为安息香中重要的总香脂酸酯类成分,与安息香生药药效有着显著相关性。
Objective： To isolate balsamic acid esters from benzoin,identify the structures and preliminarily study their efficacy. Method： Benzoin was extracted by 95% ethanol,further separated and purified by column chromatography; the isolated compounds were identified by1H-NMR and13C-NMR data. The efficacy of these isolated compounds was studied. Result： Isolated compound from benzoin was identified as coniferyl benzoate. Pharmacological experiments showed that the coniferyl benzoate and benzoin group exhibited similar effects,both of which significantly increased evans blue content in the brains of normal mice and exhibited protective effect on endotoxin fatal attack in D-galactosamine-sensitized mice. Conclusion： Coniferyl benzoate is an important component in total balsam acid esters of benzoin and has significant correlation with efficacy of crude benzoin.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第13期55-58,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重点基础研究发展计划(973计划)项目(2007CB512606)
关键词
苯甲酸松柏酯
安息香
总香脂酸
总香脂酸酯
血脑屏障
内毒素
coniferyl benzoate
benzoin
total balsamic acids
total balsamic acid esters
blood-brain barrier
endotoxin
