摘要
背景:3D打印多孔β-磷酸三钙负载聚乳酸-羟基乙酸共聚物抗结核药物缓释微球复合材料,对细胞尤其是成骨细胞生长和增殖的影响及影响程度尚缺乏可靠的生物医学证据。目的:构建3D打印多孔β-磷酸三钙负载聚乳酸-羟基乙酸共聚物抗结核药物缓释微球材料,检测其细胞毒性。方法:利用3D打印技术制备出20个孔径400μm的多孔β-磷酸三钙材料,随机抽取其中10个负载聚乳酸-羟基乙酸共聚物抗结核药物缓释微球,作为载药支架,其余10个作为非载药支架。将以上两种支架浸提液与成骨细胞共同培养72h,通过倒置相差显微镜观察细胞形态,采用CCK-8法测得成骨细胞A值,计算细胞相对增殖率,评价支架材料的细胞毒性。结果与结论:载药支架大小适度,结构规则,孔隙均匀。培养72 h内,载药支架浸提液中的成骨细胞呈长条形或长梭形,可见少量细胞核固缩,表现出轻微中毒表现,细胞毒性分级为1级,无明显细胞毒性。结果表明3D打印多孔β-磷酸三钙负载聚乳酸-羟基乙酸共聚物抗结核药物缓释微球无明显细胞毒性。
BACKGROUND： So far there is a lack of reliable biomedical evidence about the effects of three-dimensional y （3D） printed porous β-tricalcium phosphate （β-TCP） scaffold loading poly（lactic-co-glycolic acid） （PLGA）/anti-tuberculosis drug control-release microspheres on the growth and proliferation of cel s, especial y osteoblasts. OBJECTIVE： To construct porous β-TCP scaffold loading PLGA/anti-tuberculosis drug control-release microspheres by 3D printing technology and to detect its cytotoxicity. METHODS： Twenty porous β-TCP scaffolds whose aperture was 400 μm were prepared by 3D printing technology. Ten of these scaffolds were randomly selected for loading PLGA/anti-tuberculosis drug control-release microspheres, and the others were without any drugs. Then the extracts from two groups were cultured with osteoblasts for 72 hours. Afterwards, cel morphology was observed by inverted phase contrast microscope, and the absorbance value was detected using cel counting kit-8 assay. Besides, the relative growth rate of osteoblasts was calculated to evaluate the cytotoxicity of the scaffold. RESULTS AND CONCLUSION： The drug-loaded scaffold exhibited with moderate size, regular structure and uniform pores. Within 72 hours of culture in the extracts from the drug-loaded scaffold, elongated or fusiform osteoblasts appeared, with less karyopycnosis. Moreover, the drug-loaded scaffold showed slight cytotoxicity, which was classified as grade 1. In conclusion, the 3D-pinted porous β-TCP scaffold loading PLGA/anti-tuberculosis drug control-release microspheres exhibits no obvious cytotoxicity.
出处
《中国组织工程研究》
CAS
北大核心
2016年第25期3750-3756,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81371983)~~
