摘要
目的探讨医护人员MRSA定值及鼻腔mecA基因携带情况。方法随机选取本院外科、内科医护人员共200名,采集鼻试子,双靶点基因扩增技术进行NUC与mecA基因检测,对携带mecA基因的职工进行逐步Logistic回归分析。结果鼻腔MRSA定值共检出4例,占2.0%;mecA检出19例,占9.5%;内科MRSA与mecA基因阳性分别为3例与11例,分别占2.6%与9.6%,外科MRSA与mecA基因阳性分别为1例与8例,分别占1.2%与9.3%,差异均无统计学意义(P>0.05);医生组mecA基因检出率(17.5%)与护理组mecA基因检出率(5.8%)差异有统计学意义(χ2=6.78,P=0.01)。Logistic回归分析筛选出独立危险因素为未严格执行个人防护、未严格执行手卫生、所在科室未执行定值菌筛查。结论医护人员鼻腔存在MRSA定值,鼻腔mecA耐药基因检出率较高,mecA基因携带存在独立危险因素。
Objective To investigate the Methicillin -resistant Staphylococcus aureus （MRSA） colonization and carrying mecA gene in medical staff and analyze and summarize the risk factors for carrying mecA gene. Methods 200 medical staff were randomly selected from surgical department and medicine department in our hospital. Their nasal swab samples were collected for the detection of NUC gene and mecA gene by using the double target gene amplification technology. Staff carrying mecA gene were observed for the Logistic regression analysis. Results 4 cases of nasal MRSA constant value were detected, accounting for 2.0%, and 19 cases were detected for mecA gene, accounting for 9.5% ; there were 3 cases of MRSA colonization and 11 cases of positive mecA gene respectively in medical department（2.6%, 9.6% ） ; there Was 1 Case and 8 cases in surgical department （1.2% , 9.3% ） , with the difference statistically significant（P 〉 0. 05） ; the difference on the detection rate of mecA gene between in the doctor groups（ 17.5% ） and in the nursing groups（5.8% ） was statistically signiflcant（x2 = 6.78, P = 0.01 ） ; Logistic regression analysis was used to screen the independent risk factors, including not-carrying strict personal protection, not - strict implementation of hand hygiene and not - performing screening the bacteria of colonization according to the value of OR. Conclusion MRSA colonization can be detected, and the rate of mecA gene was higher in nasal cavity of medical staff. The independently dangerous factors of carrying mecA gene is existing.
出处
《中国卫生检验杂志》
CAS
2016年第12期1755-1756,1760,共3页
Chinese Journal of Health Laboratory Technology
基金
国际医学研究基金(亚洲区)临床微生物学专项基金(CNSC-J2011-A330-ZB016)
四川省卫生厅课题(120294)
内江市科技局课题(2012年)
