摘要
目的探究叶酸缺乏致大鼠巨幼细胞性贫血的相关检测指标,包括平均红细胞体积(MCV)、血象以及骨髓象。方法选取20只成年雄性SD大鼠,按照随机数字表法分为两组,分别为叶酸缺乏饲喂组-A组,叶酸正常饲喂组-B组。喂养8周,分别于实验开始及8周末测定血清叶酸、MCV,实验结束后,做外周血涂片及骨髓涂片。结果 8周末,A组血清叶酸浓度低于实验开始时,差异有统计学意义(P<0.05);A组MCV增大,中性粒细胞核分叶过多,骨髓中巨幼红细胞>10%,伴有巨幼变粒细胞及核分叶的巨核细胞。结论叶酸缺乏所致巨幼细胞性贫血,血象为大细胞正色素性贫血,血涂片中可见多数大卵圆形的红细胞,中性粒细胞分叶过多,骨髓象为骨髓呈增生活跃,红系细胞增生明显,各系细胞均有巨幼变,以红系细胞最为显著。
Objective To investigate the related detection indicators of megaloblastic anemia in rats caused by folic acid deficiency, including mean corpuscular volume（MCV）, hemogram, myelogram. Methods 20 adult male SD rats were divided into 2 groups randomly, folic acid deficient group（A group）,folic acid normal group（B group）. Detect serum folic acid and MCV at the beginning, then after 8-week feed, detect these two indicators again, and make the peripheral blood smear and bone marrow smear to observe the changes of hemogram and myelogram. Results For A group, 8 weeks later, the concentration of serum folic acid（6.05±1.67） μɡ/L） was obviously lower than the beginning index（12.76±2.03） μɡ/L）, the difference had statistically significance, MCV increased significantly, the phenomenon of hypersegmentation of neutrophil nuclei appeared, the percent of megaloblasts in bone marrow was over 10%, as well as megaloblastic change in granular leukocytes and segmented megacarycyte occurred. Conclusion For megaloblastic anemia caused by folic acid deficiency, the peripheral blood smear appeared to be more large oval red blood cells and hypersegmentation of neutrophil nuclei. The bone marrow smear showed to be active proliferation of bone marrow cells, obvious proliferation in erythroid cells, megaloblastic change in every series of cells, especially in erythroid cells.
出处
《继续医学教育》
2016年第6期139-140,共2页
Continuing Medical Education
基金
江苏省大学生创新创业训练计划基金资助项目(201410 313044Y)
