摘要
本实验研究金蝉花多糖的抗氧化活性,并对其纯化组分进行结构分析。采用热水浸提、不同浓度乙醇分级沉淀的方法从金蝉花中提取多糖,分别获得_(50)%醇沉金蝉花多糖(_(50)%polysaccharides from Cordyceps cicadas,CP_(50))和_(80)%醇沉金蝉花多糖(CP_(80)),并检测两者的体外抗氧化活性。结果表明:CP_(50)较CP_(80)表现出较强的清除自由基的能力,且具有一定的还原能力和总抗氧化能力。CP_(50)进一步经二乙氨乙基(diethylaminoethyl,DEAE)纤维素-52和Sephadex G-100凝胶柱分离纯化,得到活性多糖CPA-1和CPB-1。经紫外扫描光谱法和高效凝胶过滤色谱法鉴定CPA-1和CPB-1为均一多糖;单糖组成分析显示两个组分中均含有葡萄糖、甘露糖和半乳糖,其物质的量比分别为:1∶0.48∶0.52和1∶0.14∶0.114。红外光谱(infrared spectroscopy,IR)及刚果红实验发现,CPA-1和CPB-1具有典型的多糖红外吸收,且含有三股螺旋分子结构。
In this study, the antioxidant activities and structural characteristics of polysaccharides from Cordyceps cicadae were determined. Two crude polysaccharide fractions（CP_（50） and CP_（80）） were extracted from Cordyceps cicadae with hot water and isolated by precipitation with different concentrations of ethanol. Their antioxidant activities were also analyzed. The results indicated that both CP_（50） and CP_（80） were effective in antioxidant activity. The radical scavenging activity of CP_（50） was higher than that of CP_（80）. CP_（50） also showed higher reducing capacity and total antioxidant activity. CP_（50） was then further purified by gel filtration on diethylaminoethyl（DEAE）-cellulose-52 and Sephadex G-100 columns, and two purified fractions were obtained, namely CPA-1 and CPB-1. Ultraviolet spectroscopy and high-performance liquid chromatography（HPLC） analysis revealed that CPA-1 and CPB-1 were homogeneous. Monosaccharide analysis revealed that both CPA-1 and CPB-1 were composed of Glc, Gal and Man with a molar ratio of 1：0.48：0.52 and 1：0.14：0.114, respectively. Moreover, both polysaccharide fractions had three helix structures.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第13期19-24,共6页
Food Science
基金
国家自然科学基金面上项目(81072985
81373480
81573529)
江苏省大学生实践创新训练计划立项项目(201513986001Y)
江苏大学第13批大学生科研立项一般项目(13A154)
江苏大学第14批大学生科研立项一般项目(14A134)
关键词
金蝉花多糖
抗氧化活性
分离纯化
结构分析
Cordyceps cicadaes polysaccharides
antioxidant activity
separation and purification
structural analysis
