摘要
目的建立测定罗汉松实多糖含量的紫外分光光度法。方法采用超声辅助提取法提取多糖,以葡萄糖为对照品,采用硫酸-苯酚比色法测定罗汉松实中多糖的含量,紫外检测波长为490 nm。结果葡萄糖质量浓度在20~100μg/m L范围内与吸光度呈良好线性关系,回归方程为Y=0.008 0 X-0.015 8,r=0.998 0（n=5）,平均回收率为101.64%,RSD为4.77%（n=6）。罗汉松实多糖平均含量为4.74 mg/g（以葡萄糖计）。结论该方法操作简单,重现性好,结果准确、可靠,可用于罗汉松实多糖含量的测定。
Objective To determine the polysaccharides content of fruit from Podocarpus macrophyllus. Methods The ultrasonic-assisted extraction method was used to extract polysaccharides from the fruit of Podocarpus macrophyllus and the polysaccharides content was determined by sulphuric acid-phenol colorimetric method, with glucose as reference substance. The UV detection wavelength was 490 nm. Results Glucose absorbance showed a good linear relationship with the content in the range of 20-100 μg/mL. The regression equation is Y=0. 008 0 X-0. 0158, r=0. 998 0 （ n=5 ） , and the average recovery rate was 101. 64%, RSD=4. 77%（ n=6 ） . The aver-age content of polysaccharides in Podocarpus macrophyllus is 4. 74 mg/g （ according to glucose ） . Conclusion This determination method is simple to be operated, with good reproducibility, and the results are accurate and reliable, which can be used to determine the polysaccharides content of Podocarpus macrophyllus.
出处
《中国药业》
CAS
2016年第13期22-24,共3页
China Pharmaceuticals
基金
广西南校中青年教师基础能力提升项目
项目编号:KY2016YB096
广西南宁市生物制药产业大型科学仪器与设备共享与服务平台建设-广西医科大学共享平台建设
项目编号:20121052-1
关键词
罗汉松
多糖
含量测定
超声辅助提取
Podocarpus macrophyllus
polysaccharides
content determination
ultrasonic-assisted extraction
