摘要
重组抗狂犬病毒单克隆抗体是一种全人源的抗病毒感染单抗,主要用于狂犬病毒暴露后预防。采用糖苷酶处理2种重组抗狂犬病毒单克隆抗体Mab1、Mab2水解切除N-糖链,通过糖染色和毛细管电泳检测确定N-糖链酶解程度,利用快速荧光灶抑制试验检测其中和活性,分析N-糖链切除对该单抗中和活性的影响。结果显示,N-糖链切除后抗体中和活性无明显变化。说明N-糖链对这两株抗狂犬病毒单抗的体外中和活性不是必须的结构成分。
The recombinant anti-rabies monoclonal antibody were fully-human-derived antibody and were used for post-explosure prophylaxis of rabies virus. The N- glycan of two anti-rabies monoclonal antibodies（ Mab1 and Mab2） were hydrolyzed by the PNGase in this study,the capillary electrophoresis and gel staining by periodic acid-schiff method were used to determine the residual N-glycan after hydrolysis. The rapid fluorescent focus inhibition test was used to compare the neutralization bio-activity of the intact Mab1 and the Mab2 without N-glycan. The result showed that the neutralization bioactivity of Mab1 and Mab2 did not change after the N-glycan was removed from the antibodies. That suggests the N-glycan was not the indispensable composition for in-vitro neutralization bioactivity of Mab1 and Mab2.
出处
《生物技术进展》
2016年第4期277-282,共6页
Current Biotechnology
基金
"十二五"国家科技重大专项(2014ZX09201041)资助
关键词
重组抗狂犬病毒单克隆抗体
N-糖链切除
毛细管电泳
中和活性
recombinant anti-rabies virus monoclonal antibody
N-glycan removal
capillary electrophoresis
neutralization activity
