摘要
目的:研究自噬相关基因AKT和p62在外阴鳞癌中的表达,明确二者与外阴鳞癌临床病理特征的关系。方法:选取2008年1月至2014年12月盛京医院外阴鳞癌(VSCC)40例石蜡包埋组织作为实验组;外阴上皮内瘤变(VIN)30例、正常外阴皮肤10例石蜡包埋组织作为对照组。采用免疫组化SP法检测AKT和p62蛋白在外阴鳞癌、VIN和外阴正常皮肤中的表达水平。分析AKT、p62与VSCC临床病理特征相关性。结果:与正常外阴皮肤相比,AKT和p62蛋白在VIN中的表达显著增加,在VSCC组织中的表达量增加更为显著,与VSCC的病理分级呈正相关(P<0.05)。AKT和p62蛋白呈正相关(P<0.05)。结论:自噬相关蛋白AKT和p62在VSCC中表达增加,与VSCC的病理分级呈正相关,二者的表达改变与VSCC的发生和进展相关。
Objective：To investigate the expressions of AKT and p62 in vulva squamous cell carcinoma（VSCC） tissues,and the correlation between pathologic factors of VSCC and the expression of AKT and p62. Methods：Forty cases of VSCC were collected as experiment group,30 cases of VIN,and 10 cases of normal vulvar skin were chosen as control group in Shengjing hospital. Supervision S - P two step immunohistochemical methods were performed to de-tect the expression of AKT and p62 protein. Statistical software was applied for data analysis：the correlation between the expression of AKT and p62 proteins and VSCC was determined. Results：Compared with normal vulvar skin,the expression of AKT and p62 proteins were upregulated in VIN,and were much higher in VSCC. The expression of AKT and p62 proteins had correlation with the pathological stage of VSCC（P ﹤ 0. 05）. Moreover,there was a relationship between the expression of AKT and p62 proteins（P ﹤ 0. 05）. Conclusion：The expression of AKT and p62 proteins, which related with autophage,were up - regulated in VSCC,and had positive relationship with the pathological stage of VSCC. The changes of those two proteins correlated to the genesis and development of VSCC.
出处
《现代肿瘤医学》
CAS
2016年第16期2575-2578,共4页
Journal of Modern Oncology
基金
沈阳市科学技术项目(编号:F15-199-1-34)
关键词
外阴鳞癌
AKT
P62
自噬
AKT
vulvar squamous cell carcinoma
AKT protein
p62 protein
autophage
