摘要
目的:研究Slug与食管癌侵袭转移间的关系及其调控机制。方法:分别将构建好的pc DNA 3.1-Slug和miR-140通过Lip 2000转染到Eca-109中,以pc DNA 3.1和Lip 2000空转作为对照组,显微镜下观察转染前后细胞的形态变化,检测细胞中E-钙黏素、N-钙黏蛋白和波形蛋白的表达情况变化,另外使用穿透小室法检测细胞的侵袭能力在转染前后的变化情况。结果:转染pc DNA 3.1-Slug和miR-140到Eca-109中后细胞变得细长。转染pc DNA 3.1-Slug的Eca-109中E-钙黏素表达下调,N-钙黏蛋白和波形蛋白表达上调,细胞穿透基质胶的数量明显增多。转染miR-140的Eca-109中E-钙黏素表达上调,N-钙黏蛋白和波形蛋白表达下调,细胞穿透基质胶的数量明显减少。在食管癌细胞中miR-140和Slug的表达呈负相关(r=-0.96)。结论:转录因子Slug能够提高食管癌上皮细胞的间质转化能力和侵袭能力,而miR-140是通过调控Slug来调节食管癌细胞侵袭性的。
Objective： To study the association of Slug with invasion and metastasis of esophageal carcinoma and its regulating mechanism. Methods： pc DNA 3. 1-Slug recombinant plasmid was constructed and transfected into esophageal carcinoma cell lines of Eca-109,with pc DNA 3. 1 and Lip 2000 mock transfection served as control group. The morphological changes of cells were observed under the microscope. The expressions of E-cadherin,Ncadherin and vimentin were determined,and invasion ability of the cells before and after transfection was detected by Transwell chamber method. Results： The cells of miR-140/Eca-109 and pc DNA 3. 1-Slug/Eca-109 became elongated. The expression of E-cadherin was down-regulated,and N-cadherin and waveform protein was increased in the Eca-109 cells when transfected with pc DNA 3. 1-Slug. The expression of E-cadherin was up-regulated,and N-cadherin and waveform protein was decreased in the Eca-109 cells when transfected with miR-140. The number of cells through the Matrigel was significantly reduced. The expression of miR-140 and Slug in esophageal carcinoma cells was negatively correlated（ r =- 0. 96）. Conclusion： The Slug transcription factor can improve the ability of mesenchymal transition and invasion of esophageal epithelial cells,and miR-140 may regulate the cell invasion of Eca-109 via controlling Slug expression.
出处
《东南大学学报（医学版）》
CAS
北大核心
2016年第4期569-574,共6页
Journal of Southeast University(Medical Science Edition)
