摘要
为筛选获得甜菜耐盐相关蛋白、探讨甜菜的耐盐机理以及培育耐盐甜菜品种，采用TCA丙酮沉淀法提取经高盐（300mmoL／LNaCl）处理组和对照组的甜菜叶片蛋白，利用双向电泳对蛋白进行分离，经考马斯亮蓝染色后获得分辨率高、重复性较好的双向电泳图谱，扫描后借助软件ImageMaster2DPlatinum5．0分析蛋白质图谱。实验结果表明：共找出47个差异蛋白点，进一步分析比对，鉴定出7种，这些蛋白多数与呼吸、能量代谢及光合作用有关。
To obtain salt tolerance related proteins, exploring the mechanism of salt tolerance and cultivated salt - tolerant beet varieties ; we extracted the beet leaf protein in high salt （300 mmol/L NaC1） treatment group and control group by TCA acetone precipitation method, isolated these protein by two - dimensional electrophoresis and coomassie brilliant blue staining; after that we got the high resolution, good repeatability .two - dimensional electrophoretogram and analysis the protein map by ImageMaster 2 d Platinum 5.0. The results showed that 47 differential protein were identified and further analysis confirm 7 proteins related to respiration, energy metabo- lism and photosynthesis.
出处
《中国甜菜糖业》
2016年第2期1-3,共3页
China Beet & Sugar
基金
本研究由国家自然基金项目（31571731）、科技部技术研发专项（2013EG111232）和现代农业产业体系项目（CARS-21010202）共同资助
关键词
甜菜
盐胁迫
双向电泳
蛋白质组学
差异蛋白
Key words ： Beta vulgaris
Salt stress
Two - dimensional electrophoresis
Proteomics
Differential protein
