摘要
建立了微流控芯片非接触电导检测法快速测定盐酸克林霉素胶囊中盐酸克林霉素的方法。考察了缓冲液种类和浓度、添加剂种类、分离电压以及进样时间等因素对分离检测的影响。以2.0 mmol·L-1HAc＋2.0 mmol·L-1Na Ac为缓冲溶液,分离电压为1.5 k V,进样时间15.0 s,在1.0 min内实现了对盐酸克林霉素的快速分离测定。盐酸克林霉素的线性范围为20.0～200.0μg·m L-1,相关系数（r）为0.999 2,检出限（S/N=3）为5.0μg·m L-1,相对标准偏差为2.1%,加标回收率为95.9%～103%。该方法简便、快速,可为盐酸克林霉素检测提供新的方法和技术。
A method for the rapid determination of elindamycin hydroehloride in elindamyein hydro- chloride capsules by microchip capillary electrophoresis with contactless conductivity detection was de- veloped. Influence parameters on the separation and detection such as type and concentration of buff- er, type of additive, separation voltage and injection time were investigated. Under the optimal con- ditions, using 2. 0 mmol· L-l HAc -2.0 mmol · L-1 NaAc as buffer solution, clindamycin hydro-chloride was separated and detected within 1.0 min at a separation voltage of 1.5 kV and an injection time of 15.0 s. Clindamycin hydrochloride showed a good linear response in the range of 20. 0 - 200. 0 μg · mL-1, with a correlation coefficient（r） of 0. 999 2. The limit of detection（S/N =3）was 5.0 μg · mL-1 with RSD of 2. 1% , and the recoveries were in the range of 95.9% -103%. This method was simple and rapid, and could be used for the detection and quality control of clindamycin hydrochloride in clindamycin hydrochloride capsules.
出处
《分析测试学报》
CAS
CSCD
北大核心
2016年第7期893-896,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金(21375152)
广东省自然科学基金(21005021)
广东省科技计划项目(2014A020221098)
关键词
微流控芯片
非接触电导检测
盐酸克林霉素胶囊
盐酸克林霉素
microfluidic chip
contactless conductivity detection
clindamycin hydrochloride cap-sules
clindamycin hydrochloride
